Method for quantitative in situ hybridization histochemistry and image analysis applied for Homerla gene expression in rat brain

被引：29

作者：

Ambesi-Impiombato, A ^{[1
]}

D'Urso, G ^{[1
]}

Muscettola, G ^{[1
]}

de Bartolomeis, A ^{[1
]}

机构：

[1] Univ Naples Federico II, Lab Mol Psychiat & Psychopharmacotherapy, Sect Psychiat, Dept Neurosci & Behav Sci, I-80131 Naples, Italy

来源：

BRAIN RESEARCH PROTOCOLS | 2003年 / 11卷 / 03期

关键词：

in situ hybridization histochemistry; preprocessing; gene expression; quantitative analysis; image analysis;

D O I：

10.1016/S1385-299X(03)00056-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe the detailed method for quantitative in situ hybridization histochemistry adopted in our previously published short communication on differential gene expression of the postsynaptic density proteins Homer and PSD-95 in rat forebrain after acute treatment with antipsychotic drugs [de Bartolonneis et al., Mol. Brain Res. 98 (2002) 124-129]. Specific S-35 radiolabeled oligodeoxyribonucleotides were used to hybridize rat forebrain sections and data analysis was carried out on digitalized images acquired by means of a CCD camera. Special emphasis has been posed on data preprocessing options applied to a dataset obtained using a transparency scanner as an alternative image capturing method. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：189 / 196

页数：8

共 12 条

[1] EXPRESSION OF TYROSINE-HYDROXYLASE IN CEREBELLAR PURKINJE NEURONS OF THE MUTANT TOTTERING AND LEANER MOUSE [J].