Investigation of lipopolysaccharide receptor expression on human monocytes after major orthopaedic surgery

被引:5
作者
Bastian, D. [1 ]
Shegarfi, H. [2 ]
Rolstad, B. [2 ]
Naper, C. [3 ]
Lyngstadaas, S. P. [4 ]
Reikeras, O. [1 ]
机构
[1] Univ Oslo, Rikshosp, Radium Hosp Med Care, Dept Orthopaed,Fac Dent, NO-0027 Oslo, Norway
[2] Univ Oslo, Rikshosp, Radium Hosp Med Care, Dept Anat,Fac Dent, NO-0027 Oslo, Norway
[3] Univ Oslo, Rikshosp, Radium Hosp Med Care, Inst Immunol,Fac Dent, NO-0027 Oslo, Norway
[4] Univ Oslo, Rikshosp, Radium Hosp Med Care, Dept Biomat,Fac Dent, NO-0027 Oslo, Norway
关键词
lipopolysaccharide receptor complex; tumour necrosis factor alpha; toll-like receptor-4; CD14; monocytes;
D O I
10.1159/000112208
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: The innate immune system is suppressed after major orthopaedic surgery, implicating a risk of septic complications. Whole-blood ex vivo testing with lipopolysaccharide (LPS) has shown a depression of the tumour necrosis factor alpha (TNF-alpha) production until 12 days postoperatively. As part of the innate immune system, the Toll-like receptors TLR2 and TLR4 recognize antigens from Gram-positive and Gram-negative bacteria, respectively. The receptors CD14 and CD11b are involved in the LPS receptor complex, whereas human lymphocyte antigen (HLA)-DR binds endotoxin peptides. It is still uncertain whether the expression of all these receptors changes after major surgery. Methods: In 6 patients undergoing hip arthroplasty, we investigated three times the display of TLR4, TLR2, CD14, CD11b, and HLA-DR on monocytes by fluorescence-activated cell sorting and white blood cell counts during 12 days postoperatively. At the same time, the plasma levels of interleukin (IL)-1 beta, IL-4, IL-6, IL-10, IL-13, and TNF-alpha were measured. Results: There was no significant change in the expression of TLR4, CD14, CD11b, HLA-DR, and TLR2. Monocyte count and cytokine analysis did not differ from the ones pre-operatively taken. Conclusions: After aseptic orthopaedic surgery, there is no change in the display of the LPS receptor complex on monocytes. Other mechanisms have to be investigated to gain insight into the decrease of the TNF-alpha production capacity postoperatively. Copyright (c) 2008 S. Karger AG, Basel.
引用
收藏
页码:239 / 245
页数:7
相关论文
共 45 条
[1]
Inhibitory control of TGF-β1 on the activation of Rap1, CD11b, and transendothelial migration of leukocytes [J].
Basoni, C ;
Nobles, M ;
Grimshaw, A ;
Desgranges, C ;
Davies, D ;
Perretti, M ;
Kramer, IM ;
Genot, E .
FASEB JOURNAL, 2005, 19 (03) :822-+
[2]
Immune modulation after total hip surgery [J].
Bastian, D. ;
Sun, J. ;
Krohn, C. Danckert ;
Reikeras, O. .
EUROPEAN SURGICAL RESEARCH, 2007, 39 (05) :296-302
[3]
The proinflammatory CD14+CD16+DR++ monocytes are a major source of TNF [J].
Belge, KU ;
Dayyani, F ;
Horelt, A ;
Siedlar, M ;
Frankenberger, M ;
Frankenberger, B ;
Espevik, T ;
Ziegler-Heitbrock, L .
JOURNAL OF IMMUNOLOGY, 2002, 168 (07) :3536-3542
[4]
Interleukin-6 in the injured patient marker of injury or mediator of inflammation? [J].
Biffl, WL ;
Moore, EE ;
Moore, FA ;
Peterson, VM .
ANNALS OF SURGERY, 1996, 224 (05) :647-664
[5]
Sir Isaac Newton, sepsis, SIRS, and CARS [J].
Bone, RC .
CRITICAL CARE MEDICINE, 1996, 24 (07) :1125-1128
[6]
Brandl K, 2005, EUR J MED RES, V10, P319
[7]
Hypertonic resuscitation modulates the inflammatory response in patients with traumatic hemorrhagic shock [J].
Bulger, Eileen M. ;
Cuschieri, Joseph ;
Warner, Keir ;
Maier, Ronald V. .
ANNALS OF SURGERY, 2007, 245 (04) :635-641
[8]
Systemic and local cytokine patterns during total hip surgery [J].
Clementsen, T. ;
Krohn, C. D. ;
Reikeras, O. .
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2006, 66 (06) :535-542
[9]
The immunopathogenesis of sepsis [J].
Cohen, J .
NATURE, 2002, 420 (6917) :885-891
[10]
β2 integrins are involved in cytokine responses to whole Gram-positive bacteria [J].
Cuzzola, M ;
Mancuso, G ;
Beninati, C ;
Biondo, C ;
Genovese, F ;
Tomasello, F ;
Flo, TH ;
Espevik, T ;
Teti, G .
JOURNAL OF IMMUNOLOGY, 2000, 164 (11) :5871-5876