Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore

被引：270

作者：

Kojima, H

Hirotani, M

Nakatsubo, N

Kikuchi, K

Urano, Y

Higuchi, T

Hirata, Y

Nagano, T

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Fac Med, Dept Internal Med 2, Bunkyo Ku, Tokyo 1130033, Japan

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 09期

关键词：

Bioimaging;

D O I：

10.1021/ac001136i

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Diaminofluoresceins are widely used for detection and imaging of nitric oxide (NO), but for biological applications, they have the disadvantages that the fluorescence of the fluorescein chromophore is pH-sensitive and overlaps the autofluorescence of cells. We hac-e developed a membrane-permeable fluorescent indicator for NO based on the rhodamine chromophore, DAR-4M AM, which can be excited dth 550-nm light. The fluorescence quantum yield of the product after reaction with NO is 840 times higher than that of DAR-4M. The detection limit of NO nias 7 nM, and the fluorescence showed no pH dependency above pH 4, DAR-4M AM was successfully applied to practical bioimaging of NO produced in bovine aortic endothelial cells.

引用

页码：1967 / 1973

页数：7

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