FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly

被引:1110
作者
Webb, DJ
Donais, K
Whitmore, LA
Thomas, SM
Turner, CE
Parsons, JT
Horwitz, AF
机构
[1] UVA Sch Med, Dept Cell Biol, Charlottesville, VA 22908 USA
[2] Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USA
[3] Harvard Univ, Sch Med, Canc Biol Program, Boston, MA 02215 USA
[4] SUNY Upstate Med Univ, Dept Cell & Dev Biol, Syracuse, NY 13210 USA
关键词
D O I
10.1038/ncb1094
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cell migration is a complex, highly regulated process that involves the continuous formation and disassembly of adhesions ( adhesion turnover). Adhesion formation takes place at the leading edge of protrusions, whereas disassembly occurs both at the cell rear and at the base of protrusions. Despite the importance of these processes in migration, the mechanisms that regulate adhesion formation and disassembly remain largely unknown. Here we develop quantitative assays to measure the rate of incorporation of molecules into adhesions and the departure of these proteins from adhesions. Using these assays, we show that kinases and adaptor molecules, including focal adhesion kinase (FAK), Src, p130CAS, paxillin, extracellular signal-regulated kinase (ERK) and myosin light-chain kinase (MLCK) are critical for adhesion turnover at the cell front, a process central to migration.
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页码:154 / +
页数:11
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