Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery

被引：28

作者：

Shi, Jian ^{[1
]}

Li, Yuan ^{[2
]}

Liang, Shuzhen ^{[2
]}

Zeng, Jianying ^{[2
]}

Liu, Guifeng ^{[2
]}

Mu, Feng ^{[2
]}

Li, Haibo ^{[2
]}

Chen, Jibing ^{[2
]}

Liu, Tongjun ^{[1
]}

Niu, Lizhi ^{[2
]}

机构：

[1] Jilin Univ, Dept Gen Surg, Hosp 2, Changchun, Jilin, Peoples R China

[2] Jinan Univ, Guangzhou Fuda Canc Inst, Fuda Canc Hosp, Sch Med, Guangzhou, Guangdong, Peoples R China

来源：

CANCER BIOLOGY & THERAPY | 2016年 / 17卷 / 09期

关键词：

Circulating tumor cells; cryosurgery; colorectal cancer; flow cytometry; liver metastasis; reverse transcriptase polymerase chain reaction; BREAST-CANCER; DISEASE PROGRESSION; PERIPHERAL-BLOOD; LUNG-CANCER; FOLLOW-UP; SURVIVAL; DISSEMINATION; CRYOABLATION; CRYOTHERAPY; COMBINATION;

D O I：

10.1080/15384047.2016.1210731

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

引用

页码：935 / 942

页数：8

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