A high-throughput soft agar assay for identification of anticancer compound

被引：33

作者：

Anderson, Steven N. ^{[1
]}

Towne, Danli L. ^{[1
]}

Burns, David J. ^{[1
]}

Warrior, Usha ^{[1
]}

机构：

[1] Abbott Labs, Global Pharmaceut Res & Dev, Dept Biol Screening, Abbott Pk, IL USA

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2007年 / 12卷 / 07期

关键词：

soft agar; HTS; 3-D; anoikis; HCC827;

D O I：

10.1177/1087057107306130

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A 384-well soft agar assay was developed to identify potential novel anticancer compounds. Normally used to detect cell transformation, the assay is used here to quantitate cell proliferation in a 3-dimensional (3-D) anchorage-independent format. HCC827 cells, which are highly sensitive to epithelial growth factor receptor (EGFR) tyrosine kinase inhibitors, were used to develop the method and a set of 9600 compounds used to validate the assay. Results were compared to a monolayer assay using the same compound set. The assay provides a robust method to discover compounds that could be missed using traditional monolayer formats.

引用

页码：938 / 945

页数：8

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