Upregulation of PKC delta- and downregulation of PKC alpha-mRNA and protein by a phorbol ester in human T84 cells

被引：23

作者：

Assert, R ^{[1
]}

Schatz, H ^{[1
]}

Pfeiffer, A ^{[1
]}

机构：

[1] UNIV KLIN BOCHUM,BERUFSGENOSSENSCHAFTLICHE KLINIKEN BERGMANNSHEIL,MED KLIN & POLIKLIN,D-44789 DRESDEN,GERMANY

来源：

FEBS LETTERS | 1996年 / 388卷 / 2-3期

关键词：

protein kinase C delta; protein kinase C regulation; protein kinase C mRNA regulation;

D O I：

10.1016/0014-5793(96)00522-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein kinase C (PKC) family members are downregulated by chronic activation at the protein level in most cell types. In T84 human epithelial cells 12-O-tetradecanoyl phorbol 13-acetate (TPA) caused persistent translocation of PKC delta to the membrane compartment and a 400% increase of PKC delta-mRNA after 24 h. In contrast, PKC alpha protein was completely downregulated and its mRNA was decreased to 60% of control levels after 24 h. This is the first report of PKC delta-mRNA upregulation by TPA which was previously only shown for PKC beta. In view of the antimitogenic actions of PKC delta this pattern of regulation may serve to preserve growth control even in the presence of chronic cell activation.

引用

页码：195 / 199

页数：5

共 28 条

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