Neural circuitry of capsaicin-sensitive afferents innervating submucosal arterioles in guinea pig ileum

The circuitry of capsaicin-sensitive nerves innervating submucosal arterioles in the guinea pig ileum mas examined. The orientation of in vitro submucosal preparations in a double-chamber bath was varied so that nerves on differing segments of arterioles could be stimulated with capsaicin. Capsaicin-evoked dilation of preconstricted arterioles was recorded using videomicroscopy. Superfusion of capsaicin onto either proximal or distal segments of a parent arteriole divided between the chambers evoked a dilation in the opposite chamber (63 and 58%, respectively) but had no effect on extrinsically denervated preparations. When the divider separated the vascular arcades joining the two parent arterioles on the opposite or same side of the intestine, capsaicin evoked little or no response (3 and 11%, respectively). Capsaicin stimulation confined to one branch of a single vessel dilated the opposite branch (42%). In preparations with adjacently attached mucosa, application of capsaicin to the mucosa dilated arterioles in the apposite chamber. These findings suggest that capsaicin stimulation of the mucosa evokes dilation of arterioles through a submucosal reflex and that both afferent and efferent elements are confined to the submucosa and mucosa.