Cloning and sequence analysis of a lysophospholipase homologue from Schistosoma mansoni

被引：7

作者：

Hamdan, FF ^{[1
]}

Ribeiro, P ^{[1
]}

机构：

[1] McGill Univ, Inst Parasitol, St Anne De Bellevue, PQ H9X 3V9, Canada

来源：

PARASITOLOGY RESEARCH | 1998年 / 84卷 / 10期

关键词：

D O I：

10.1007/s004360050497

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A novel cDNA encoding a lysophospholipase (SmLPLH) homologue has been cloned from the human parasite, Schistosoma mansoni. The predicted primary structure of SmLPLH shares high sequence homology with mammalian lysophospholipases (62%) and also contains aconsensus signature peptide (GXSXG), which is present in all lipases. Two splice variants of SmLPLH that differ by 32 N-terminal amino acids were identified. The shorter truncated species carries a conserved S. mansoni spliced leader (SL) sequence at its 5' end and is believed to be formed by trans-splicing of the SL to an internal exon splice site. The functional significance of these two isoforms of SmLPLH is currently unknown.

引用

页码：839 / 842

页数：4

共 16 条

[1] THE MOLECULAR EVOLUTION OF GENES AND PROTEINS - A TALE OF 2 SERINES [J].

BRENNER, S .

NATURE, 1988, 334 (6182) :528-530

[2] Gene linkage and steady state RNAs suggest trans-splicing may be associated with a polycistronic transcript in Schistosoma mansoni [J].

Davis, RE ;

Hodgson, S .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 89 (01) :25-39

[3] RNA TRANSSPLICING IN FLATWORMS - ANALYSIS OF TRANS-SPLICED MESSENGER-RNAS AND GENES IN THE HUMAN PARASITE, SCHISTOSOMA-MANSONI [J].

DAVIS, RE ;

HARDWICK, C ;

TAVERNIER, P ;

HODGSON, S ;

SINGH, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (37) :21813-21819

[4] Surprising diversity and distribution of spliced leader RNAs in flatworms [J].

Davis, RE .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 87 (01) :29-48

[5] SCHISTOSOMA-MANSONI - SYNTHESIS AND RELEASE OF PHOSPHOLIPIDS, LYSOPHOSPHOLIPIDS, AND NEUTRAL LIPIDS BY SCHISTOSOMULA [J].

FURLONG, ST ;

CAULFIELD, JP .

EXPERIMENTAL PARASITOLOGY, 1989, 69 (01) :65-77

[6] SCHISTOSOMA-MANSONI - STEROL AND PHOSPHOLIPID-COMPOSITION OF CERCARIAE, SCHISTOSOMULA, AND ADULTS [J].

FURLONG, ST ;

CAULFIELD, JP .

EXPERIMENTAL PARASITOLOGY, 1988, 65 (02) :222-231

[7] SCHISTOSOMULA OF SCHISTOSOMA-MANSONI USE LYSOPHOSPHATIDYLCHOLINE TO LYSE ADHERENT HUMAN RED-BLOOD-CELLS AND IMMOBILIZE RED-CELL MEMBRANE-COMPONENTS [J].

GOLAN, DE ;

BROWN, CS ;

CIANCI, CML ;

FURLONG, ST ;

CAULFIELD, JP .

JOURNAL OF CELL BIOLOGY, 1986, 103 (03) :819-828

[8] MONOPALMITOYLPHOSPHATIDYLCHOLINE INCORPORATION INTO HUMAN-ERYTHROCYTE GHOST MEMBRANES CAUSES PROTEIN AND LIPID IMMOBILIZATION AND CHOLESTEROL DEPLETION [J].

GOLAN, DE ;

FURLONG, ST ;

BROWN, CS ;

CAULFIELD, JP .

BIOCHEMISTRY, 1988, 27 (08) :2661-2667

[9] CHARACTERIZATION OF PSEUDOMONAS-FLUORESCENS CARBOXYLESTERASE - CLONING AND EXPRESSION OF THE ESTERASE GENE IN ESCHERICHIA-COLI [J].

HONG, KH ;

JANG, WH ;

CHOI, KD ;

YOO, OJ .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (11) :2839-2845

[10] A SPLICED LEADER IS PRESENT ON A SUBSET OF MESSENGER-RNAS FROM THE HUMAN PARASITE SCHISTOSOMA-MANSONI [J].

RAJKOVIC, A ;

DAVIS, RE ;

SIMONSEN, JN ;

ROTTMAN, FM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (22) :8879-8883

← 1 2 →