The morphology and composition of influenza A virus particles are not affected by low levels of M1 and M2 proteins in infected cells

被引：31

作者：

Bourmakina, SV ^{[1
]}

García-Sastre, A ^{[1
]}

机构：

[1] CUNY Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 12期

关键词：

D O I：

10.1128/JVI.79.12.7926-7932.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We generated a recombinant influenza A virus (Mmut) that produced low levels of matrix (M1) and M2 proteins in infected cells. Mmut virus propagated to significantly lower titers than did wild-type virus in cells infected at low multiplicity. By contrast, virion morphology and incorporation of viral proteins and vRNAs into virus particles were similar to those of wild-type virus. We propose that a threshold amount of M1 protein is needed for the assembly of viral components into an infectious particle and that budding is delayed in Mmut virus-infected cells until sufficient levels of M1 protein accumulate at the plasma membrane.

引用

页码：7926 / 7932

页数：7

共 55 条

[1] Influenza virus assembly: Effect of influenza virus glycoproteins on the membrane association of M1 protein [J].

Ali, A ;

Avalos, RT ;

Ponimaskin, E ;

Nayak, DP .

JOURNAL OF VIROLOGY, 2000, 74 (18) :8709-8719

[2] Sequence of the 1918 pandemic influenza virus nonstructural gene (NS) segment and characterization of recombinant viruses hearing the 1918 NS genes [J].

Basler, CF ;

Reid, AH ;

Dybing, JK ;

Janczewski, TA ;

Fanning, TG ;

Zheng, HY ;

Salvatore, M ;

Perdue, ML ;

Swayne, DE ;

García-Sastre, A ;

Palese, P ;

Taubenberger, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (05) :2746-2751

[3] In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein [J].

Baudin, F ;

Petit, I ;

Weissenhorn, W ;

Ruigrok, RWH .

VIROLOGY, 2001, 281 (01) :102-108

[4] Reverse genetics studies on the filamentous morphology of influenza A virus [J].

Bourmakina, SV ;

García-Sastre, A .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :517-527

[5] Alternative base pairs attenuate influenza A virus when introduced into the duplex region of the conserved viral RNA promoter of either the NS or the PA gene [J].

Catchpole, AP ;

Mingay, LJ ;

Fodor, E ;

Brownlee, GG .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :507-515

[6] A matrix-less measles virus is infectious and elicits extensive cell fusion: consequences for propagation in the brain [J].

Cathomen, T ;

Mrkic, B ;

Spehner, D ;

Drillien, R ;

Naef, R ;

Pavlovic, J ;

Aguzzi, A ;

Billeter, MA ;

Cattaneo, R .

EMBO JOURNAL, 1998, 17 (14) :3899-3908

[7] A novel influenza A virus mitochondrial protein that induces cell death [J].

Chen, WS ;

Calvo, PA ;

Malide, D ;

Gibbs, J ;

Schubert, U ;

Bacik, I ;

Basta, S ;

O'Neill, R ;

Schickli, J ;

Palese, P ;

Henklein, P ;

Bennink, JR ;

Yewdell, JW .

NATURE MEDICINE, 2001, 7 (12) :1306-1312

[8] Human parainfluenza virus type 1 matrix and nucleoprotein genes transiently expressed in mammalian cells induce the release of virus-like particles containing nucleocapsid-like structures [J].

Coronel, EC ;

Murti, KG ;

Takimoto, T ;

Portner, A .

JOURNAL OF VIROLOGY, 1999, 73 (08) :7035-7038

[9] The M1 matrix protein controls the filamentous phenotype of influenza A virus [J].

Elleman, CJ ;

Barclay, WS .

VIROLOGY, 2004, 321 (01) :144-153

[10] Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway [J].

Elton, D ;

Simpson-Holley, M ;

Archer, K ;

Medcalf, L ;

Hallam, R ;

McCauley, J ;

Digard, P .

JOURNAL OF VIROLOGY, 2001, 75 (01) :408-419

← 1 2 3 4 5 6 →