The effects of inflammatory cytokines on steroidogenic acute regulatory protein expression in macrophages

被引：39

作者：

Ma, Y. ^{[1
]}

Ren, S. ^{[2
]}

Pandak, W. M. ^{[2
]}

Li, X. ^{[1
]}

Ning, Y. ^{[1
]}

Lu, C. ^{[1
]}

Zhao, F. ^{[1
]}

Yin, L. ^{[1
]}

机构：

[1] Fudan Univ, Shaghai Med Coll, Dept Physiol & Pathophysiol, Shanghai 200032, Peoples R China

[2] Virginia Commonwealth Univ, VA Med Ctr, Dept Med, Richmond, VA 23298 USA

来源：

INFLAMMATION RESEARCH | 2007年 / 56卷 / 12期

基金：

中国国家自然科学基金;

关键词：

steroidogenic acute regulatory protein; tumor necrosis factor-alpha; interferon-gamma; transforming growth factor-beta1;

D O I：

10.1007/s00011-007-6133-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Objective: To investigate the expression of steroidogenic acute regulatory protein (StAR) in macrophages and the effects of inflammatory cytokines on StAR expression. Methods: The macrophages isolated from ApoE knockout mice and C57BL/6J mice and RAW264.7 cells (a cell line from mouse macrophage. ATCC Number: TIB-71 (TM)) were cultured in DMEM containing 10% fetal bovine serum. RAW264.7 cells were treated with different inflammatory cytokines (TNF-alpha, IFN-gamma and TGF-beta 1) and 8-Br-cAMP, a cAMP analog. RT-PCR and Western blot analysis were applied to evaluate the effects of inflammatory cytokines on StAR expression. Results: RT-PCR and Western blot analysis demonstrated the expression of StAR in the macrophages isolated from ApoE knockout mice, C57BL/6J mice and RAW264.7 cells. Pro-inflammatory cytokines TNF-alpha and IFN-gamma significantly decreased StAR mRNA and protein levels in RAW264.7 cells. The inhibition was dose- and time-dependent. In contrast, anti-inflammatory cytokine TGF-beta 1 increased StAR mRNA and protein levels. At 1: 15 molecular ratio, TGF-beta 1 blocked the down-regulation of StAR expression mediated by TNF-alpha. cAMP also induced StAR expression in RAW264.7 cells. When the cells were co-treated with 8-Br-cAMP and TNF-alpha, 8-Br-cAMP failed to induce StAR expression. Conclusion: Our results provide interesting evidence that inflammatory cytokines regulate StAR expression in macrophages.

引用

页码：495 / 501

页数：7

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