Differential Anti-APOBEC3G Activity of HIV-1 Vif Proteins Derived from Different Subtypes

被引：49

作者：

Iwabu, Yukie

Kinomoto, Masanobu

Tatsumi, Masashi ^{[2
]}

Fujita, Hideaki ^{[3
]}

Shimura, Mari ^{[4
]}

Tanaka, Yoshitaka ^{[3
]}

Ishizaka, Yukihito ^{[4
]}

Nolan, David ^{[5
,6
]}

Mallal, Simon ^{[5
,6
]}

Sata, Tetsutaro

Tokunaga, Kenzo ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Pathol, Shinjuku Ku, Tokyo 1628640, Japan

[2] Natl Inst Infect Dis, AIDS Res Ctr, Tokyo 1628640, Japan

[3] Kyushu Univ, Grad Sch Pharmaceut Sci, Div Pharmaceut Cell Biol, Fukuoka 8128582, Japan

[4] Int Med Ctr Japan, Dept Intractable Dis, Tokyo 1628655, Japan

[5] Royal Perth Hosp, Ctr Clin Immunol & Biomed Stat, Perth, WA 6000, Australia

[6] Murdoch Univ, Perth, WA 6000, Australia

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 46期

关键词：

HUMAN-IMMUNODEFICIENCY-VIRUS; CD4(+) T-CELLS; HUMAN APOBEC3G; TYPE-1; VIF; ANTIVIRAL ACTIVITY; LINE-1; RETROTRANSPOSITION; MONONUCLEAR PHAGOCYTES; ANTIRETROVIRAL FACTOR; RETROVIRAL INFECTION; CYTIDINE DEAMINASES;

D O I：

10.1074/jbc.M110.173286

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antiretroviral cytidine deaminase APOBEC3G, which is abundantly expressed in peripheral blood lymphocytes and macrophages, strongly protects these cells against HIV-1 infection. The HIV-1 Vif protein overcomes this antiviral effect by enhancing proteasome-mediated APOBEC3G degradation and is key for maintaining viral infectivity. The 579-bp-long vif gene displays high genetic diversity among HIV-1 subtypes. Therefore, it is intriguing to address whether Vif proteins derived from different subtypes differ in their viral defense activity against APOBEC3G. Expression plasmids encoding Vif proteins derived from subtypes A, B, C, CRF01_AE, and CRF02_AG isolates were created, and their anti-APOBEC3G activities were compared. Viruses produced from cells expressing APOBEC3G and Vif proteins from different subtypes showed relatively different viral infectivities. Notably, subtype C-derived Vif proteins tested had the highest activity against APOBEC3G that was ascribed to its increased binding activity, for which the N-terminal domain of the Vif protein sequences was responsible. These results suggest that the biological differences of Vif proteins belonging to different subtypes might affect viral fitness and quasispecies in vivo.

引用

页码：35350 / 35358

页数：9

共 82 条

[1] CCR5-and CXCR4-Tropic Subtype C Human Immunodeficiency Virus Type 1 Isolates Have a Lower Level of Pathogenic Fitness than Other Dominant Group M Subtypes: Implications for the Epidemic [J].