In vivo characteristics and localisation of carotenoid pigments in psychrotrophic and mesophilic Micrococcus roseus using photoacoustic spectroscopy

被引:14
作者
Jagannadham, MV [1 ]
Narayanan, K [1 ]
Rao, CM [1 ]
Shivaji, S [1 ]
机构
[1] CTR CELLULAR & MOL BIOL,HYDERABAD 500007,ANDHRA PRADESH,INDIA
关键词
D O I
10.1006/bbrc.1996.1493
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Photoacoustic spectra of cells of M.roseus were recorded to study the in vivo characteristics and localisation of the carotenoid pigments in these cells. The PA spectra indicated that both the psychrotrophic and mesophilic strains had similar chromophores. The cis carotenoids were prominent in the psychrotrophic M.roseus whereas shorter polyenes were more prominent in mesophilic M.roseus. Further, depth profiling photoacoustic studies revealed that in both the strains of M.roseus the bulk of the chromophore was associated with the cell membrane. (C) 1996 Academic Press, Inc.
引用
收藏
页码:221 / 226
页数:6
相关论文
共 29 条
[1]   ANALYTICAL OPTOACOUSTIC SPECTROMETRY .3. OPTOACOUSTIC EFFECT AND THERMAL-DIFFUSIVITY [J].
ADAMS, MJ ;
KIRKBRIGHT, GF .
ANALYST, 1977, 102 (1213) :281-292
[2]   A PHOTOACOUSTIC DEPTH PROFILE OF BETA-CAROTENE IN SKIN [J].
ANJO, DM ;
MOORE, TA .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1984, 39 (05) :635-640
[3]  
[Anonymous], PHYSL PLANT
[4]   ACTION OF VISIBLE LIGHT ON ENZYMES IN CELL ENVELOPES OF MICROCOCCUS-ROSEUS [J].
ASCENZI, JM ;
COONEY, JJ .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1975, 21 (05) :307-311
[5]   THE MALARIA PARASITE MONITORED BY PHOTOACOUSTIC-SPECTROSCOPY [J].
BALASUBRAMANIAN, D ;
RAO, CM ;
PANIJPAN, B .
SCIENCE, 1984, 223 (4638) :828-830
[6]   PHOTO-ACOUSTIC SPECTROSCOPY OF ANACYSTIS-NIDULANS .1. EFFECT OF SAMPLE THICKNESS ON THE PHOTO-ACOUSTIC SIGNAL [J].
CARPENTIER, R ;
LARUE, B ;
LEBLANC, RM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 222 (02) :403-410
[7]  
CHAUHAN S, 1994, POLAR BIOL, V14, P31
[8]  
COONEY JJ, 1966, J BACTERIOL, V92, P341
[9]  
Davies BH, 1976, CHEMISTRY BIOCHEMIST, P38, DOI DOI 10.1590/S0101-20612001000200017
[10]  
GOODWIN TW, 1988, PLANT PIGMENTS, P1