Gangliosides derived from a T cell lymphoma inhibit bone marrow cell proliferation and differentiation

被引:21
作者
Bharti, AC [1 ]
Singh, SM [1 ]
机构
[1] Banaras Hindu Univ, Sch Biotechnol, Varanasi 221005, Uttar Pradesh, India
关键词
Dalton's lymphoma; gangliosides; bone marrow; hematopoiesis; immunosuppression; myeloid differentiation; lymphocyte proliferation;
D O I
10.1016/S1567-5769(00)00004-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Previously, we have observed a modulation in the hone marrow; hematopoiesis and alteration in the repertoire of blood monocytes and lymphocytes in mice bearing a spontaneous T cell lymphoma, designated as Dalton's lymphoma (DL). In the present investigation, we show that in vivo or in vitro treatment of bone marrow cells (BMC) with gangliosides of DL (DLG) results in inhibition of proliferative ability and alteration of colony-forming ability (CFA) of BMC. BMC incubated with DLG also showed a decrease in cell viability in a concentration-dependent manner. BMC colony-forming assays in the presence of macrophage-colony-stimulating factor (M-CSF) showed a dose-dependent decrease in the number of colonies with a concomitant decrease in macrophage- and granulocyte macrophage-colony-forming units (CFU-M and CFU-GM, respectively) and an increase in granulocyte-CFU (CFU-G). Neuraminidase treatment of DLG abrogated their inhibitory action on BMC. Further, antibodies to G(D3) and to lesser extent G(M2) neutralized the inhibitory effect of DLG on BMC. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:155 / 165
页数:11
相关论文
共 42 条
[1]   One hundred years of cancer immunotherapy: A critical appraisal [J].
Ben-Efraim, S .
TUMOR BIOLOGY, 1999, 20 (01) :1-24
[2]   Induction of apoptosis in bone marrow cells by gangliosides produced by a T cell lymphoma [J].
Bharti, AC ;
Singh, SM .
IMMUNOLOGY LETTERS, 2000, 72 (01) :39-48
[3]  
BREMER EG, 1984, J BIOL CHEM, V259, P6818
[4]  
CHU JWK, 1995, IMMUNOLOGY, V84, P396
[5]  
DEGOWIN RL, 1978, EXP HEMATOL, V6, P568
[6]   Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis [J].
DeMaria, R ;
Lenti, L ;
Malisan, F ;
dAgostino, F ;
Tomassini, B ;
Zeuner, A ;
Rippo, MR ;
Testi, R .
SCIENCE, 1997, 277 (5332) :1652-1655
[7]  
DEXTER TM, 1987, ANNU REV CELL BIOL, V3, P423
[8]  
Dozmorov IM, 1997, BIOCHEM MOL BIOL INT, V42, P57
[9]  
DUMONTET C, 1994, CANCER IMMUNOL IMMUN, V38, P311, DOI 10.1007/s002620050071
[10]   INFLUENCE OF EXTRACELLULAR MATRICES ON GANGLIOSIDE PATTERN OF 2 HEPATOMA-CELL LINES WITH DIFFERENT ADHESIVE PROPERTIES [J].
FRITSCH, M ;
GEILEN, CC ;
HEIDRICH, C ;
REUTTER, W .
FEBS LETTERS, 1995, 376 (03) :159-163