Xenon does not affect human platelet function in vitro

被引:22
作者
de Rossi, LW
Horn, NA
Baumert, JH
Gutensohn, K
Hutschenreuter, G
Rossaint, R
机构
[1] Univ Hosp, Dept Anesthesiol, Rhein Westfal TH Aachen, D-52074 Aachen, Germany
[2] Univ Hamburg, Hosp Eppendorf, Dept Transfus Med Transplantat Immunol, D-20246 Hamburg, Germany
[3] Univ Hosp, Inst Transfus Med, Rhein Westfal TH Aachen, D-52074 Aachen, Germany
关键词
D O I
10.1097/00000539-200109000-00020
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
We sought to determine whether xenon affects platelet glycoprotein expression and platelet-related hemostasis in vitro at a clinically relevant concentration. Human whole blood was stimulated with either adenosine diphosphate or the thrombin receptor agonist peptide (TRAP)-6 after incubation with 65% xenon. Halothane at 2 minimum alveolar anesthetic concentration was used as a positive control. Platelet function and activation were evaluated with two-color flow cytometry. The expression of the platelet glycoproteins GPIIb/IIIa, GPIb, and P selectin were detected with fluorochrome-conjugated monoclonal antibodies. In vitro measurement of platelet-related hemostasis under conditions of high shear stress was performed in citrated whole blood with a platelet function analyzer (PFA-100 (R)) by using collagen/epinephrine and collagen/adenosine diphosphate cartridges. Xenon did not affect basal or agonist-induced expression of platelet membrane glycoproteins, activation-dependent conformational. changes of the GPIIb/IIIa receptor, expression of P selectin, or PFA closure times. In contrast, halothane reduced TRAP-6-induced activation of the GPIIb/IIIa complex. Furthermore, collagen/epinephrine-induced PFA closure time was significantly prolonged. These results demonstrate that xenon does not affect the unstimulated or agonist-induced platelet glycoprotein expression, activation of GPIIb/IIIa, or platelet-related hemostasis.
引用
收藏
页码:635 / 640
页数:6
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