Partial characterization of a Sendai virus replication promoter and the rule of six

被引:54
作者
Pelet, T [1 ]
Delenda, C [1 ]
Gubbay, O [1 ]
Garcin, D [1 ]
Kolakofsky, D [1 ]
机构
[1] UNIV GENEVA,SCH MED,CMU,DEPT GENET & MICROBIOL,CH-1211 GENEVA,SWITZERLAND
关键词
D O I
10.1006/viro.1996.0547
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have used a cDNA copy of a natural, internally deleted, Sendai virus defective interfering genome to study the effect of insertions and deletions (which maintain the hexamer genome length) on the ability of viral genomes to be amplified in a transfected cell system. The insertion of 18 nt at nt(72) (in the 5' untranslated region of the N gene, just downstream of the le(+) region) was found to be lethal, whereas similar insertions further from the genome ends were well tolerated. Curiously, the insertion of 6 nt on either side of the le(+)/N junction (at nt(47) and nt(67)) was well tolerated, but the insertion of 12 nt at either site, or of 6 nt at both sites, largely ablated genome amplification. These results suggest that an element of this replication promoter is located downstream of nt(67), in the 5' untranslated region of the first gene. Remarkably, the addition of 6 nt by the insertion of 2, 3, or 4 nt at nt(47) plus the insertion of 4, 3, or 2 nt, respectively, at nt(67) was poorly tolerated, presumably because the hexamer phase of the intervening sequence was altered with respect to the N subunits of the template. These results suggest that the rule of six operates, at least in part, at the level of the initiation of antigenome synthesis. (C) 1996 Academic Press, Inc.
引用
收藏
页码:405 / 414
页数:10
相关论文
共 38 条
[11]   MUTATIONS IN CONSERVED DOMAIN-I OF THE SENDAI-VIRUS L-POLYMERASE PROTEIN UNCOUPLE TRANSCRIPTION AND REPLICATION [J].
CHANDRIKA, R ;
HORIKAMI, SM ;
SMALLWOOD, S ;
MOYER, SA .
VIROLOGY, 1995, 213 (02) :352-363
[12]  
CHAREST PM, UNPUB
[13]   THE HIV-1 TAT PROTEIN - AN RNA SEQUENCE-SPECIFIC PROCESSIVITY FACTOR [J].
CULLEN, BR .
CELL, 1990, 63 (04) :655-657
[14]   THE SENDAI VIRUS P-GENE EXPRESSES BOTH AN ESSENTIAL PROTEIN AND AN INHIBITOR OF RNA-SYNTHESIS BY SHUFFLING MODULES VIA MESSENGER-RNA EDITING [J].
CURRAN, J ;
BOECK, R ;
KOLAKOFSKY, D .
EMBO JOURNAL, 1991, 10 (10) :3079-3085
[15]   THE SENDAI VIRUS NUCLEOCAPSID EXISTS IN AT LEAST 4 DIFFERENT HELICAL STATES [J].
EGELMAN, EH ;
WU, SS ;
AMREIN, M ;
PORTNER, A ;
MURTI, G .
JOURNAL OF VIROLOGY, 1989, 63 (05) :2233-2243
[16]   FROM INITIATION TO ELONGATION - COMPARISON OF TRANSCRIPTION BY PROKARYOTIC AND EUKARYOTIC RNA-POLYMERASES [J].
EICK, D ;
WEDEL, A ;
HEUMANN, H .
TRENDS IN GENETICS, 1994, 10 (08) :292-296
[17]   MOLECULAR-CLONING AND CHARACTERIZATION OF A SENDAI VIRUS INTERNAL DELETION DEFECTIVE-RNA [J].
ENGELHORN, M ;
STRICKER, R ;
ROUX, L .
JOURNAL OF GENERAL VIROLOGY, 1993, 74 :137-141
[18]   EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].
FUERST, TR ;
NILES, EG ;
STUDIER, FW ;
MOSS, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126
[19]   A highly recombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA: Generation of a novel copy-back nondefective interfering virus [J].
Garcin, D ;
Pelet, T ;
Calain, P ;
Roux, L ;
Curran, J ;
Kolakofsky, D .
EMBO JOURNAL, 1995, 14 (24) :6087-6094
[20]   MUTATIONS WITHIN NONCODING TERMINAL SEQUENCES OF MODEL RNAS OF SENDAI VIRUS - INFLUENCE ON REPORTER GENE-EXPRESSION [J].
HARTY, RN ;
PALESE, P .
JOURNAL OF VIROLOGY, 1995, 69 (08) :5128-5131