Tumour-related enzyme alterations in the clear cell type of human renal cell carcinoma identified by two-dimensional gel electrophoresis

被引：69

作者：

Balabanov, S

Zimmermann, U

Protzel, C

Scharf, C

Klebingat, KJ

Walther, R ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Inst Med Biochem & Mol Biol, D-17489 Greifswald, Germany

[2] Ernst Moritz Arndt Univ Greifswald, Dept Urol, D-17489 Greifswald, Germany

[3] Ernst Moritz Arndt Univ Greifswald, Dept Mol Biol & Microbiol, D-17489 Greifswald, Germany

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 22期

关键词：

renal cell carcinoma (RCC); 2D-gel electrophoresis; kidney; proteome; enzyme alteration;

D O I：

10.1046/j.0014-2956.2001.02546.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify tumour-related enzyme alterations we have used 2D-gels to analyse the proteome from dissected malignant and benign kidney areas from patients with clear-cell-type renal carcinoma. The expression of 12 proteins was diminished in tumour. Four proteins were characterized by mass spectrometry and were identified as enoyl-CoA hydratase, alpha -glycerol-3-phosphate dehydrogenase, aldehyde dehydrogenase 1 and aminoacylase-1.

引用

页码：5977 / 5980

页数：4

共 18 条

[1] SEQUENTIAL-CHANGES IN GLYCOGEN-CONTENT, EXPRESSION OF GLUCOSE TRANSPORTERS AND ENZYMATIC PATTERNS DURING DEVELOPMENT OF CLEAR ACIDOPHILIC CELL TUMORS IN RAT-KIDNEY [J].