Fipronil resistance in Sogatella furcifera: Molecular cloning and functional expression of wild-type and mutant RDL GABA receptor subunits

被引：45

作者：

Nakao, Toshifumi ^{[1
]}

Hama, Masako ^{[1
]}

Kawahara, Nobuyuki ^{[1
]}

Hirase, Kangetsu ^{[1
]}

机构：

[1] Mitsui Chem Agro Inc, Agrochem Res Ctr, Mobara, Chiba 2970017, Japan

来源：

JOURNAL OF PESTICIDE SCIENCE | 2012年 / 37卷 / 01期

关键词：

GABA receptor; whitebacked planthopper; Sogatella furcifera; fipronil resistance; GAMMA-AMINOBUTYRIC-ACID; GATED CHLORIDE CHANNELS; LAODELPHAX-STRIATELLUS; INSECTICIDE RESISTANCE; DIELDRIN RESISTANCE; DROSOPHILA; MUTATION; IDENTIFICATION; BINDING; PROTEIN;

D O I：

10.1584/jpestics.D11-018

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The whitebacked planthopper Sogatella furcifera is a serious pest of rice that has developed fipronil resistance in Asia. We found a novel R340Q mutation in the cytoplasmic loop between M3 and M4 of the S. furcifera RDL gamma-aminobutyric acid (GABA) receptor subunit (SF-RDL). The R340Q mutation was found in clones carrying the A2'N mutation, which was previously implicated in fipronil resistance and suggested to be a heterozygous mutation. To investigate the influence of mutations, Drosophila Mel-2 cells were transfected with wild-type or mutant SF-RDL genes, either individually or together. A membrane potential assay showed that the influence of the A2'N.R340Q double mutation on fipronil resistance was more profound than that of the A2'N single mutation in the heterozygous expression of wild-type and mutant SF-RDL genes. (C) Pesticide Science Society of Japan

引用

页码：37 / 44

页数：8

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