FRAP analysis of binding: proper and fitting

被引:378
作者
Sprague, BL [1 ]
McNally, JG [1 ]
机构
[1] NCI, Lab Receptor Biol & Gene Express, Bethesda, MD 20892 USA
关键词
D O I
10.1016/j.tcb.2004.12.001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Dynamic molecular interactions are fundamental to all cellular processes. In vivo analyses of these interactions are frequently done using fluorescence recovery after photobleaching (FRAP). Proper interpretation of FRAP data yields information about the binding interactions of fluorescently tagged molecules, including the number of binding states and the binding strength of each state. This binding information can be gleaned from appropriate models of the process underlying a FRAP recovery. Continued application and development of these approaches promise to provide crucial information for a quantitative description of the molecular networks that regulate cellular function.
引用
收藏
页码:84 / 91
页数:8
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