Life style and urinary 8-hydroxydleoxyguanosine, a marker of oxidative DNA damage: Effects of exercise, working conditions, meat intake, body mass index, and smoking

被引:141
作者
Kasai, H [1 ]
Iwamoto-Tanaka, N
Miyamoto, T
Kawanami, K
Kawanami, S
Kido, R
Ikeda, M
机构
[1] Univ Occupat & Environm Hlth, Inst Ind Ecol Sci, Dept Environm Oncol, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan
[2] Univ Occupat & Environm Hlth, Inst Ind Ecol Sci, Dept Occupat Hlth Econ, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan
[3] Nippon Steel Corp Ltd, Tech Dev Bur, Kimitsu 2991141, Japan
来源
JAPANESE JOURNAL OF CANCER RESEARCH | 2001年 / 92卷 / 01期
关键词
8-hydroxydeoxyguanosine; exercise; meat intake; BMI; smoking;
D O I
10.1111/j.1349-7006.2001.tb01041.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The urinary levels of 8-hydroxydeoxyguanosine (8-OH-dG), a marker of oxidative DNA damage, of 318 healthy men aged 18-58 were measured with high resolution by a newly developed automated high-pressure liquid chromatography (HPLC) system coupled to an electrochemical detector (ECD). The mean 8-OH-dG level (mug/g creatinine) was 4.12+/-1.73 (SD). An eleven-fold inter-individual variation was observed. The accuracy of the measurement estimated from the recovery of an added 8-OH-dG standard was 90-98%. By univariate analysis, it was found that moderate physical exercise (P=0.0023) and high body mass index (BMI) (P=0.0032) reduced the 8-OH-dG level, while physical labor (P=0.0097), smoking (P=0.032), and low meat intake (less than once/week) (P=0.041) increased its level. Based on a multi-regression analysis of the log-transformed values, moderate physical exercise (P=0.0039), high BMI (P=0.0099), and age (P=0.021) showed significant reducing effects on the 8-OH-dG level, while low meat intake (P=0.010), smoking (P=0.013), and day-night shift work (P=0.044) increased its level. These results suggest that many types of life-style factors that either generate or scavenge oxygen radicals may affect the level of oxidative DNA damage of each individual.
引用
收藏
页码:9 / 15
页数:7
相关论文
共 23 条
[1]   Effects of forced and spontaneous exercise on 8-hydroxydeoxyguanosine levels in rat organs [J].
Asami, S ;
Hirano, T ;
Yamaguchi, R ;
Tsurudome, Y ;
Itoh, H ;
Kasai, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 243 (03) :678-682
[2]   Cigarette smoking induces an increase in oxidative DNA damage, 8-hydroxydeoxyguanosine, in a central site of the human lung [J].
Asami, S ;
Manabe, H ;
Miyake, J ;
Tsurudome, Y ;
Hirano, T ;
Yamaguchi, R ;
Itoh, H ;
Kasai, H .
CARCINOGENESIS, 1997, 18 (09) :1763-1766
[3]  
Baik SC, 1996, CANCER RES, V56, P1279
[4]   A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2 '-deoxyguanosine measurements in urine and other biologic matrices: A one-year evaluation of methods [J].
Bogdanov, MB ;
Beal, MF ;
McCabe, DR ;
Griffin, RM ;
Matson, WR .
FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (5-6) :647-666
[5]   REACTIONS OF OXYL RADICALS WITH DNA [J].
BREEN, AP ;
MURPHY, JA .
FREE RADICAL BIOLOGY AND MEDICINE, 1995, 18 (06) :1033-1077
[6]  
CHENG KC, 1992, J BIOL CHEM, V267, P166
[7]  
CUTLER RG, 1991, ANN NY ACAD SCI, V621, P1
[8]  
Floyd R A, 1986, Free Radic Res Commun, V1, P163, DOI 10.3109/10715768609083148
[9]   FREE-RADICALS, ANTIOXIDANTS, AND HUMAN-DISEASE - CURIOSITY, CAUSE, OR CONSEQUENCE [J].
HALLIWELL, B .
LANCET, 1994, 344 (8924) :721-724
[10]   Diet, physical activity and cancer risk [J].
Hill, Michael J. .
PUBLIC HEALTH NUTRITION, 1999, 2 (3A) :397-401