Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

被引：12

作者：

Alberto, F ^{[1
]}

Correia, L

Billot, C

Duarte, CM

Serrao, E

机构：

[1] Univ Algarve, FCMA, Ctr Marine Sci, CCMAR, P-8005139 Faro, Portugal

[2] UIB, CSIC, IMEDEA, Inst Mediterraneo Estudios Avanzados, Esporles 07190, Mallorca, Spain

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 03期

关键词：

clonal plant; Cymodocea nodosa; microsatellites; seagrass;

D O I：

10.1046/j.1471-8286.2003.00463.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.

引用

页码：397 / 399

页数：3

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