Computer identification of Shigella species by rRNA gene restriction patterns

被引：8

作者：

Coimbra, RS ^{[1
]}

Nicastro, G ^{[1
]}

Grimont, PAD ^{[1
]}

Grimont, F ^{[1
]}

机构：

[1] Inst Pasteur, Inserm 389, Unite Enterobacteries, F-75724 Paris 15, France

来源：

RESEARCH IN MICROBIOLOGY | 2001年 / 152卷 / 01期

关键词：

ribotyping; Shigella; molecular epidemiology; molecular typing; serotyping;

D O I：

10.1016/S0923-2508(00)01167-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a MluI ribotyping scheme for Shigella which approaches correlation with serotyping. One hundred and seventeen reference strains and previously serotyped clinical isolates representing the 57 Shigella serotypes and biotypes were included in this study. A total of 51 distinct ribotypes were obtained and a database was built with them. The number of bands composing each ribotype varied from 9 to 15. The fragments ranged in size from 1.6 to 18.8 kbp. One hundred and eleven clinical isolates were successfully identified in a double blind study with standard biochemical/serologic methods, by automatic comparison of their ribotypes with our database using the software Taxotron. (C) 2001 Editions scientifiques ct medicales Elsevier SAS.

引用

页码：47 / 55

页数：9

共 31 条

[1]

ANSARUZZAMAN M, 1995, J CLIN MICROBIOL, V3, P1423

[2] MOLECULAR EPIDEMIOLOGY UNRAVELS THE COMPLEXITY OF NEONATAL ESCHERICHIA-COLI ACQUISITION IN TWINS [J].

BINGEN, EH ;

DENAMUR, E ;

PICARD, B ;

GOULLET, P ;

LAMBERTZECHOVSKY, NY ;

BRAHIMI, N ;

MERCIER, JC ;

BEAUFILS, F ;

ELION, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1896-1898

[3] POLYNUCLEOTIDE SEQUENCE RELATEDNESS AMONG SHIGELLA SPECIES [J].

BRENNER, DJ ;

FANNING, GR ;

MIKLOS, GV ;

STEIGERWALT, AG .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1973, 23 (01) :1-7

[4] THE OAC GENE ENCODING A LIPOPOLYSACCHARIDE O-ANTIGEN ACETYLASE MAPS ADJACENT TO THE INTEGRASE-ENCODING GENE ON THE GENOME OF SHIGELLA-FLEXNERI BACTERIOPHAGE-SF6 [J].

CLARK, CA ;

BELTRAME, J ;

MANNING, PA .

GENE, 1991, 107 (01) :43-52

[5] Identification of Shigella serotypes by restriction of amplified O-antigen gene cluster [J].

Coimbra, RS ;

Grimont, F ;

Grimont, PAD .

RESEARCH IN MICROBIOLOGY, 1999, 150 (08) :543-553

[6]

COIMBRA RS, 2000, IN PRESS RES MICROBI

[7] SEROLOGY OF SHIGELLA [J].

EWING, WH ;

LINDBERG, AA .

METHODS IN MICROBIOLOGY, 1984, 14 :113-142

[8]

EWING WH, 1986, IDENTIFICATION ENTER, P133

[9] DIFFERENTIATION OF SHIGELLA-FLEXNERI STRAINS BY RIBOSOMAL-RNA GENE RESTRICTION PATTERNS [J].

FARUQUE, SM ;

HAIDER, K ;

RAHMAN, MM ;

ALIM, ARMA ;

AHMAD, QS ;

ALBERT, MJ ;

SACK, RB .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) :2996-2999

[10]

GIAMMANCO G, 1969, ANN I PASTEUR PARIS, V117, P16

← 1 2 3 4 →