Inhibition of NF-kappa B activation by panepoxydone

被引:114
作者
Erkel, G [1 ]
Anke, T [1 ]
Sterner, O [1 ]
机构
[1] LUND UNIV,CTR CHEM,DIV ORGAN CHEM 2,S-22100 LUND,SWEDEN
关键词
D O I
10.1006/bbrc.1996.1335
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In a search for new inhibitors of NF-kappa B mediated signal transduction in COS-7 cells using the Secreted Alkaline Phosphatase as reporter gene, panepoxydone has been isolated from fermentations of the basidiomycete Lentinus crinitus. Panepoxydone inhibits the NF-kappa B activated expression of the SEAP with an IC50 of 1.5 - 2 mu g/ml (7.15 - 9.52 mu M). NO inhibition of AP-1 mediated expression of the reporter gene could be observed at a concentration up to 5 mu g/ml panepoxydone. Panepoxydone strongly reduced the TPA, TNF-alpha and ocadaic acid mediated binding of NF-kappa B to the high affinity consensus sequence in COS-7 and HeLa S3 cells as confirmed by EMSA's. Panepoxydone inhibits the I kappa B phosphorylation and therfore sequesteres the NF-kappa B complex in an inactive form. (C) 1996 Academic Press, Inc.
引用
收藏
页码:214 / 221
页数:8
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