Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells

被引：85

作者：

Lee, JK

Bussey, KJ

Gwadry, FG

Reinhold, W

Riddick, G

Pelletier, SL

Nishizuka, S

Szakacs, G

Annereau, JP

Shankavaram, U

Lababidi, S

Smith, LH

Gottesman, MM

Weinstein, JN

机构：

[1] NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[2] NCI, Mol Pharmacol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

来源：

GENOME BIOLOGY | 2003年 / 4卷 / 12期

关键词：

D O I：

10.1186/gb-2003-4-12-r82

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient.

引用

页数：12

共 27 条

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