Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus

被引:47
作者
Dearnaley, JDW [1 ]
Daggard, GA [1 ]
机构
[1] Univ So Queensland, Dept Biol & Phys Sci, Toowoomba, Qld 4350, Australia
来源
SEXUAL PLANT REPRODUCTION | 2001年 / 13卷 / 05期
关键词
Brassica napus; pollen tubes; stigma; pollination; polygalacturonase;
D O I
10.1007/s004970000062
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Penetration of pollen tubes through stigmatic tissues in Brassica napus L, may involve the release of cell wall modifying enzymes from the pollen tube tip. We examined the expression of a pectin-degrading polygalacturonase (PG) enzyme in unpollinated and early and late pollinated stigmas via immunoblotting and immuno-light microscopy using a PG polyclonal antibody. Immunoblotting analysis indicated that PG enzyme was present at low levels in unpollinated stigmas and at high levels in pollinated stigmas. The level of PG did not detectably increase between early and late pollinated stigmas. Immune-light microscopy demonstrated that PG enzyme was present in ungerminated pollen grains, stigmatic papillae and in the tip of pollen tubes growing into the papillar wall. This latter evidence suggests that PG enzyme may play an important role in papillar cell wall penetration during pollination although other interpretations of the role of pollen PG should not be discounted.
引用
收藏
页码:265 / 271
页数:7
相关论文
共 32 条
[1]   A GENE SHOWING SEQUENCE SIMILARITY TO PECTIN ESTERASE IS SPECIFICALLY EXPRESSED IN DEVELOPING POLLEN OF BRASSICA-NAPUS - SEQUENCES IN ITS 5' FLANKING REGION ARE CONSERVED IN OTHER POLLEN-SPECIFIC PROMOTERS [J].
ALBANI, D ;
ALTOSAAR, I ;
ARNISON, PG ;
FABIJANSKI, SF .
PLANT MOLECULAR BIOLOGY, 1991, 16 (04) :501-513
[2]   REPRESSION OF THE HIGH-METHIONINE ZEIN GENE IN THE MAIZE INBRED LINE MO17 [J].
SCHICKLER, H ;
BENNER, MS ;
MESSING, J .
PLANT JOURNAL, 1993, 3 (02) :221-229
[3]   Inhibition of polygalacturonase activity produced by Leptosphaeria maculans with stem extracts of canola, and the relationship of inhibition to resistance [J].
Annis, SL ;
Goodwin, PH .
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT, 1997, 145 (5-6) :217-223
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   CHARACTERIZATION OF A GENE FAMILY ABUNDANTLY EXPRESSED IN OENOTHERA-ORGANENSIS POLLEN THAT SHOWS SEQUENCE SIMILARITY TO POLYGALACTURONASE [J].
BROWN, SM ;
CROUCH, ML .
PLANT CELL, 1990, 2 (03) :263-274
[6]   Neither compatible nor self-incompatible pollinations of Brassica napus involve reorganization of the papillar cytoskeleton [J].
Dearnaley, JDW ;
Clark, KM ;
Heath, IB ;
Lew, RR ;
Goring, DR .
NEW PHYTOLOGIST, 1999, 141 (02) :199-207
[7]  
DICKINSON H, 1995, SEX PLANT REPROD, V8, P1, DOI 10.1007/BF00228756
[8]  
Dircks LK, 1996, PLANT PHYSIOL BIOCH, V34, P509
[9]   THE UBIQUITOUS PRESENCE OF EXOPOLYGALACTURONASE IN MAIZE SUGGESTS A FUNDAMENTAL CELLULAR FUNCTION FOR THIS ENZYME [J].
DUBALD, M ;
BARAKATE, A ;
MANDARON, P ;
MACHE, R .
PLANT JOURNAL, 1993, 4 (05) :781-791
[10]   INTERACTION BETWEEN THE POLLEN-TUBE AND STIGMATIC CELL-WALL FOLLOWING POLLINATION IN BRASSICA-OLERACEA [J].
ELLEMAN, CJ ;
WILLSON, CE ;
SARKER, RH ;
DICKINSON, HG .
NEW PHYTOLOGIST, 1988, 109 (01) :111-117