A tetra-primer amplification refractory mutation system polymerase chain reaction for the evaluation of rs12979860 IL28B genotype

被引：22

作者：

Galmozzi, E. ^{[1
]}

Del Menico, B. ^{[1
]}

Rametta, R. ^{[1
]}

Dongiovanni, P. ^{[1
]}

Fracanzani, A. L. ^{[1
]}

Benedan, L. ^{[1
]}

Borroni, V. ^{[1
]}

Maggioni, P. ^{[1
]}

Fargion, S. ^{[1
]}

Valenti, L. ^{[1
]}

机构：

[1] Univ Milan, Osped Maggiore, Policlin Ca Granda IRCCS, Dept Internal Med,Ctr Malattie Metabol Fegato, I-20122 Milan, Italy

来源：

JOURNAL OF VIRAL HEPATITIS | 2011年 / 18卷 / 09期

关键词：

chronic hepatitis C; genetics; IL28; interferon lambda; single nucleotide polymorphism; treatment response; GENETIC-VARIATION; CLEARANCE; IL28B;

D O I：

10.1111/j.1365-2893.2010.01349.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Recently, genome-wide association studies in patients affected by HCV infection have identified a strong association between sustained virological response to peg-interferon/ribavirin and spontaneous viral clearance and common single nucleotide polymorphisms (SNPs) near the IL28B gene, encoding for interferon-lambda-3. Thus, it is anticipated that IL28B genotype determination will be integrated in clinical practice to guide treatment decisions. Here, we describe a simple tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR) for the evaluation of the rs12979860 C>T IL28B SNP, for which strong evidence of association with clinical outcomes has been collected in subjects of European descent. Valid genotypic data were obtained for over 99% of subjects analysed, and T-ARMS-PCR procedures were validated by the analysis of DNA samples of 164 patients with chronic HCV infection. In conclusion, this method allows rapid, reproducible, inexpensive and accurate detection of rs12979860 polymorphism without need of any special equipment and is also suitable for evaluation of a low number of samples on a routine basis.

引用

页码：628 / 630

页数：3

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