Superhydrophobic-Superhydrophilic Micropatterning: Towards Genome-on-a-Chip Cell Microarrays

被引：219

作者：

Geyer, Florian L. ^{[1
,3
]}

Ueda, Erica ^{[1
]}

Liebel, Urban ^{[1
]}

Grau, Nicole ^{[2
]}

Levkin, Pavel A. ^{[1
,3
]}

机构：

[1] Karlsruhe Inst Technol, Inst Toxicol & Genet, Karlsruhe, Germany

[2] Heidelberg Univ, Med Fac Mannheim, D-6900 Heidelberg, Germany

[3] Heidelberg Univ, Dept Appl Phys Chem, D-6900 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 36期

关键词：

cell microarrays; cell transfection; high-throughput screening; polymers; superhydrophobicity; REVERSE TRANSFECTION; SURFACE; ARRAYS; POLYMERS;

D O I：

10.1002/anie.201102545

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

High-density cell microarrays based on superhydrophilic microspots separated by superhydrophobic barriers have been realized. The microspots absorb water solutions, while the barriers prevent cross-contamination, thus allowing the spots to be used as reservoirs for transfection mixtures and preventing cell proliferation and cell migration between the microspots. The picture shows four cell types after two days of culturing on the microarray. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8424 / 8427

页数：4

共 18 条

[1] Microarrays of small molecules embedded in biodegradable polymers for use in mammalian cell-based screens [J].

Bailey, SN ;

Sabatini, DM ;

Stockwell, BR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (46) :16144-16149

[2] Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates [J].

Erfle, Holger ;

Neumann, Beate ;

Rogers, Phill ;

Bulkescher, Jutta ;

Ellenberg, Jan ;

Pepperkok, Rainer .

JOURNAL OF BIOMOLECULAR SCREENING, 2008, 13 (07) :575-580

[3] Reverse transfection on cell arrays for high content screening microscopy [J].

Erfle, Holger ;

Neumann, Beate ;

Liebel, Urban ;

Rogers, Phill ;

Held, Michael ;

Walter, Thomas ;

Ellenberg, Jan ;

Pepperkok, Rainer .

NATURE PROTOCOLS, 2007, 2 (02) :392-399

[4] An extracellular matrix microarray for probing cellular differentiation [J].

Flaim, CJ ;

Chien, S ;

Bhatia, SN .

NATURE METHODS, 2005, 2 (02) :119-125

[5] Microarray technology: beyond transcript profiling and genotype analysis [J].

Hoheisel, JD .

NATURE REVIEWS GENETICS, 2006, 7 (03) :200-210

[6] Surface manilpulation of biomolecules for cell microarray applications [J].

Hook, Andrew L. ;

Thissen, Helmut ;

Voelcker, Nicolas H. .

TRENDS IN BIOTECHNOLOGY, 2006, 24 (10) :471-477

[7] Porous Polymer Coatings: a Versatile Approach to Superhydrophobic Surfaces [J].

Levkin, Pavel A. ;

Svec, Frantisek ;

Frechet, Jean M. J. .

ADVANCED FUNCTIONAL MATERIALS, 2009, 19 (12) :1993-1998

[8]

Li XM, 2007, CHEM SOC REV, V36, P1350, DOI 10.1039/b602486f

[9] Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes [J].

Neumann, Beate ;

Walter, Thomas ;

Heriche, Jean-Karim ;

Bulkescher, Jutta ;

Erfle, Holger ;

Conrad, Christian ;

Rogers, Phill ;

Poser, Ina ;

Held, Michael ;

Liebel, Urban ;

Cetin, Cihan ;

Sieckmann, Frank ;

Pau, Gregoire ;

Kabbe, Rolf ;

Wuensche, Annelie ;

Satagopam, Venkata ;

Schmitz, Michael H. A. ;

Chapuis, Catherine ;

Gerlich, Daniel W. ;

Schneider, Reinhard ;

Eils, Roland ;

Huber, Wolfgang ;

Peters, Jan-Michael ;

Hyman, Anthony A. ;

Durbin, Richard ;

Pepperkok, Rainer ;

Ellenberg, Jan .

NATURE, 2010, 464 (7289) :721-727

[10]

Perrimon N, 2007, GENETICS, V175, P7, DOI 10.1534/genetics.106.069963

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