Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma

被引:38
作者
Shafer, RW [1 ]
Levee, DJ [1 ]
Winters, MA [1 ]
Richmond, KL [1 ]
Huang, D [1 ]
Merigan, TC [1 ]
机构
[1] RUSH PRESBYTERIAN ST LUKES MED COLL,VIROL QUAL ASSURANCE LAB,DEPT IMMUNOL MICROBIOL,CHICAGO,IL
关键词
D O I
10.1128/JCM.35.2.520-522.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human immunodeficiency virus type 1 (HIV-1) pol mutations are responsible for HIV-1 resistance to current antiretroviral drugs. HIV-1 RNA extraction with QIAamp HCV kit spin columns (Qiagen, Chatsworth, Calif.) followed by reverse transcription-PCR successfully recovered a 1,008-bp pol fragment from the plasma of 31 of 34 HIV-1-infected patients that was suitable for sequencing and recombinant-virus studies. The minimum HTV-1 RNA concentration required for gene recovery was 30 to 40 copies/ml, which was similar to the minimal HIV-1 RNA concentration required when phenol-chloroform or silica beads are used for RNA extraction.
引用
收藏
页码:520 / 522
页数:3
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