Bacterial lipopolysaccharides induce genes involved in the innate immune response in embryos of the zebrafish (Danio rerio)

被引：100

作者：

Watzke, Joerg ^{[1
]}

Schirmer, Kristin ^{[1
]}

Scholz, Stefan ^{[1
]}

机构：

[1] UFZ Helmholtz Ctr Environm Res, Helmholtz Ctr Environm Res, Dept Cell Toxicol, D-04318 Leipzig, Germany

来源：

FISH & SHELLFISH IMMUNOLOGY | 2007年 / 23卷 / 04期

关键词：

innate immunity; zebrafish; embryo; TNF-alpha; IL-1; beta; NF-kappa B; gene expression; environmental chemicals; chorion;

D O I：

10.1016/j.fsi.2007.03.004

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The innate immune response in fish represents an early, rapid defence against pathogens. Environmental contaminants could disturb this defence and negatively influence the ability to protect against infection. However, analysis of immune-modulation has not yet been included in testing strategies for environmental risk assessment of chemicals. In order to establish an efficient, small scale test system, the ability to induce the innate immune response by bacterial lipopolysaccharides in zebrafish embryos was investigated. The level of expression of various genes involved in inflammation was used as the endpoint. We could show that immersion of embryos in LPS induced the gene expression of two key pro-inflammatory cytokines, tumor necrosis factor a and interleukin 10 in 32 h old zebrafish embryos. The gene induction required the removal of the chorion prior to lipopolysaccharide exposure. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：901 / 905

页数：5

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