Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens

被引：24

作者：

Kong, FR

Gordon, S

Gilbert, GL

机构：

[1] Westmead Hosp, Ctr Infect Dis & Microbiol, Inst Clin Pathol & Med Res, Westmead, NSW 2145, Australia

[2] Royal Alexandra Hosp Children, Natl Ctr Immunisat Res & Surveillnace Vaccine Pre, Westmead, NSW 2145, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 11期

关键词：

D O I：

10.1128/JCM.38.11.4256-4259.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 was identified in 3 and type 2 in 7.

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页码：4256 / 4259

页数：4

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