Efficient real-time confocal microscopy with white light sources

被引:72
作者
Juskaitis, R [1 ]
Wilson, T [1 ]
Neil, MAA [1 ]
Kozubek, M [1 ]
机构
[1] UNIV OXFORD,DEPT ENGN SCI,OXFORD OX1 3PJ,ENGLAND
关键词
D O I
10.1038/383804a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
THE main advantage of confocal microscopes over their conventional counterparts arises from their ability to optically 'section' nearly transparent materials; the thin image slices thus obtained can be used to reconstruct three-dimensional images, a capability which is particularly useful for the study of biological specimens, Confocal microscopes have previously used either a single laser-illuminated point-source and single point-detector (which are scanned in tandem across the object) or white-light illumination with multiple point-sources and detectors, Single-point-source systems, however, do not usually form images in real time and are restricted to using available laser wavelengths, Multiple-point-source systems, on the other hand, produce images in real time but use light very inefficiently-typically 1% or less is used for imaging, Here we demonstrate a white-light, multiple-point-source method which can in principle produce images in real time,vith light efficiencies as high as 50%. This system is likely to find broad practical application, particularly in the imaging of weakly reflecting or weakly fluorescent specimens.
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页码:804 / 806
页数:3
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