EMF genes interact with late-flowering genes in regulating floral initiation genes during shoot development in Arabidopsis thaliana

To investigate the mechanisms regulating the initiation of floral development in Arabidopsis, a construct containing beta -glucuronidase (GUS) gene driven by APETALA1 promoter (AP1::GUS) was introduced into emf fwa and emf ft double mutants. GUS activity was strongly detected on shoot meristem of emf1-1 Single mutants harboring AP1:GUS construct just 5 d after germination. By contrast, GUS activity was undetectable on emf1-1 fwa-1, emf1-1 ft-1, emf2-1 fwa-1, emf2-3 fwa-1 and emf2-3 ft-1 double mutants harboring API::GUS construct 10 d after germination, GUS activity was only weakly detected on the apical meristem of 20-day-old emf1-1 fwa-1 and emf2-1 fn,a-l seedlings. During this time, only sessile leaves were produced. Further analysis indicated that API was strongly expressed in 10-day-old emf1-1 and emf2-1 Single mutants. Its expression was significantly reduced in all emf1-1 or emf2-1 late-flowering double mutants tested. Similar to API, the expression of LEA FY(LFY) was also high in emf1-1 and emf2-1 single mutants and reduced in emf1-1 or emf2-1 late-flowering double mutants. Our results indicate that the precocious expression of API and LFY is dependent not only on the low EMF and FWA activities but also on the expression of most of the late-flowering genes such as FT, FCA, FE, CO and GI,These data also reveal that most late-flowering genes may function downstream of EMF or in pathways distinct from EI)IF to activate genes specified floral meristem identity during shoot maturation in Arabidopsis.