Analysis of expression sequence tags from Nicotiana sylvestris

被引：6

作者：

Katoh, A

Yamaguchi, Y

Sano, H

Hashimoto, T ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Res & Educ Ctr Genet Informat, Nara 6300192, Japan

[2] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 2003年 / 79卷 / 06期

关键词：

tobacco; expression sequence tags; database;

D O I：

10.2183/pjab.79B.151

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Partially normalized complementary DNA (cDNA) libraries were generated from root and leaf tissues of a diploid tobacco species, Nicotiana sylvestris. Single-pass sequences were obtained from a total of 13,019 cDNA clones and were assembled into 6,513 non-redundant cDNA groups. We estimate that these cDNA sequences represent approximately 3,500 unique tobacco genes. The EST database described here will become a valuable resource for future research using Nicotiana species.

引用

收藏

页码：151 / 154

页数：4

相关论文

共 13 条

[1]

Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P211, DOI DOI 10.1007/BF02672069

[2] Genome-wide expression analysis of plant cell cycle modulated genes [J].

Breyne, P ;

Zabeau, M .

CURRENT OPINION IN PLANT BIOLOGY, 2001, 4 (02) :136-142

[3] Transcriptome analysis during cell division in plants [J].

Breyne, P ;

Dreesen, R ;

Vandepoele, K ;

De Veylder, L ;

Van Breusegem, F ;

Callewaert, L ;

Rombauts, S ;

Raes, J ;

Cannoot, B ;

Engler, G ;

Inzé, D ;

Zabeau, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14825-14830

[4] Alkaloid biosynthesis in plants: Biochemistry, cell biology, molecular regulation, and metabolic engineering applications [J].

Facchini, PJ .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2001, 52 :29-66

[5] A bright future for the bright yellow-2 cell culture [J].

Geelen, DNV ;

Inzé, DG .

PLANT PHYSIOLOGY, 2001, 127 (04) :1375-1379

[6] Intraspecific variability of the tandem repeats in Nicotiana putrescine N-methyltransferases [J].

Hashimoto, T ;

Shoji, T ;

Mihara, T ;

Oguri, H ;

Tamaki, K ;

Suzuki, K ;

Yamada, Y .

PLANT MOLECULAR BIOLOGY, 1998, 37 (01) :25-37

[7] ALKALOID BIOGENESIS - MOLECULAR ASPECTS [J].

HASHIMOTO, T ;

YAMADA, Y .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1994, 45 :257-285

[8] CHARACTERIZATION OF THE NICOTIANA-TABACUM-L GENOME BY MOLECULAR CYTOGENETICS [J].

KENTON, A ;

PAROKONNY, AS ;

GLEBA, YY ;

BENNETT, MD .

MOLECULAR & GENERAL GENETICS, 1993, 240 (02) :159-169

[9]

NAGEL R, 1990, FEMS MICROBIOL LETT, V67, P325, DOI 10.1111/j.1574-6968.1990.tb04041.x

[10] TOBACCO SINGLE-COPY DNA IS HIGHLY HOMOLOGOUS TO SEQUENCES PRESENT IN THE GENOMES OF ITS DIPLOID PROGENITORS [J].

OKAMURO, JK ;

GOLDBERG, RB .

MOLECULAR & GENERAL GENETICS, 1985, 198 (02) :290-298