HPLC analysis of anomeric formation and cleavage pattern by chitinolytic enzyme

被引：68

作者：

Koga, D ^{[1
]}

Yoshioka, K

Arakane, Y

机构：

[1] Yamaguchi Univ, Fac Agr, Biochem Lab, Yamaguchi 7538515, Japan

[2] Bankaku Sohonpo, Res Lab, Aichi 4760003, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 08期

关键词：

chitinase; lysozyme; chitinolytic enzyme; N-acetylchitooligosaccharide;

D O I：

10.1271/bbb.62.1643

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reactions of N-acetylchitooligosaccharides with chitinolytic enzyme were analyzed by HPLC using a Tosoh TSK-Gel amide-80 column with 70% acetonitrile as an eluent. We separated alpha and beta anomeric forms of N-acetyl-chitooligosaccharides, and obtain the following advantages of this HPLC method. 1. We can easily identify the reaction mechanism of chitinolytic enzymes by this method, distinguishing the inverting mechanism showing alpha anomer formation from the retaining mechanism showing beta anomer formation. 2. We can also estimate the cleavage patterns of N-acetylchitooligosaccharides by chitinolytic enzymes by using natural substrates.

引用

页码：1643 / 1646

页数：4

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