Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts

被引：99

作者：

Bresnahan, WA ^{[1
]}

Hultman, GE ^{[1
]}

Shenk, T ^{[1
]}

机构：

[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

来源：

JOURNAL OF VIROLOGY | 2000年 / 74卷 / 22期

关键词：

D O I：

10.1128/JVI.74.22.10816-10818.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

引用

页码：10816 / 10818

页数：3

共 14 条

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