Demonstration of UDP-glucose dehydrogenase activity in cell extracts of Escherichia coli expressing the pneumococcal cap3A gene required for the synthesis of type 3 capsular polysaccharide

被引：29

作者：

Arrecubieta, C ^{[1
]}

Garcia, E ^{[1
]}

Lopez, R ^{[1
]}

机构：

[1] CSIC, CTR INVEST BIOL, DEPT MOLEC MICROBIOL, E-28006 MADRID, SPAIN

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 10期

关键词：

D O I：

10.1128/jb.178.10.2971-2974.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene cluster of Streptococcus pneumoniae coding for the type 3 capsular polysaccharide contains four genes (cap3ABCD). A DNA fragment containing the cap3A gene was amplified by PCR and cloned under the control of a T7 RNA polymerase-dependent promoter. Overexpression of this gene in Escherichia coli resulted both in a 47-kDa protein in the cytoplasm of isopropyl-beta-D-thiogalactopyranoside-induced bacteria and in high levels of UDP-glucose dehydrogenase activity. These data demonstrate, in a direct experimental way, that cap3A encodes the UDP-glucose dehydrogenase of pneumococcus type 3.

引用

页码：2971 / 2974

页数：4

共 23 条

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