Optimization and validation of post-column assay for screening of radical scavengers in herbal raw materials and herbal preparations

被引：33

作者：

Raudonis, Raimondas ^{[1
]}

Bumblauskiene, Lina ^{[1
]}

Jakstas, Valdas ^{[1
]}

Pukalskas, Audrius ^{[2
]}

Janulis, Valdimaras ^{[1
]}

机构：

[1] Kaunas Univ Med, Dept Pharmacognosy, Kaunas, Lithuania

[2] Kaunas Univ Technol, Dept Food Technol, Kaunas, Lithuania

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 49期

关键词：

Post-column; On line HPLC ABTS; Antioxidant capacity; Perilla; ANTIOXIDANT ACTIVITY; NATURAL ANTIOXIDANTS; ROSMARINIC ACID; ONLINE; FLAVONOIDS; CATION; POLYPHENOLS; EXTRACTS; IDENTIFICATION; COMPONENTS;

D O I：

10.1016/j.chroma.2010.10.017

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

On-line method which combines HPLC distribution and post-column reaction was designed for the search of individual antioxidants Optimization of the assay was performed evaluating optimal ABTS(+) radical cation concentration in the reactor reaction time impact of flow rate reaction coil length HPLC-ABTS assay validation in this work was performed by assessing reference antioxidant negative peak areas in radical scavenging chromatogram Sample free radical scavenging activity is expressed as trolox equivalent antioxidant capacity (TEAC) Optimized and validated method was applied in detection of compounds possessing free radical scavenging ability in complex mixtures Antioxidant compounds were studied in perilla (Perilla frutescens (L) Britton var crispa f viridis) herbal raw material and its preparations The HPLC-separated antioxidant compounds were identified using HPLC-photodiode array coupled to mass spectrometer using a reference mass for determining accurate masses Radical scavenging characteristics of rosmarinic acid which is the dominant phenolic compound in medicinal herbal raw material of perilla and its preparations were confirmed by the calculated TEAC values Compounds responsible for antioxidant effect in herbal raw materials and herbal preparations were identified evaluated and compared (C) 2010 Elsevier B V All rights reserved

引用

页码：7690 / 7698

页数：9

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