Effect of adrenomedullin on ion transport and muscle contraction in rat distal colon

被引：16

作者：

Fukuda, K ^{[1
]}

Tsukada, H ^{[1
]}

Onomura, M ^{[1
]}

Saito, T ^{[1
]}

Kodama, M ^{[1
]}

Nakamura, H ^{[1
]}

Taniguchi, T ^{[1
]}

Tominaga, M ^{[1
]}

Hosokawa, M ^{[1
]}

Seino, Y ^{[1
]}

机构：

[1] Kyoto Univ, Fac Med, Dept Metab & Clin Nutr, Sakyo Ku, Kyoto 6068507, Japan

来源：

PEPTIDES | 1998年 / 19卷 / 06期

关键词：

D O I：

10.1016/S0196-9781(98)00043-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the effects of adrenomedullin (AM) on mucosal ion transport using the Ussing method and smooth muscle contraction using the Magnus method in rat. Our results indicate that AM increases Isc (short-circuit current) for Cl- secretion (100 nM:170.0 +/- 41.2%, 1 mu M:193.8 +/- 45.5%, 100% Isc:28.2 +/- 3.1 mu A/cm(2)) with an intial small decrease of Isc, inhibiting Na+ absorption. Tetrodotoxin (TTX) inhibits the Isc response elicited by AM (86%). In addition, AM relaxes potassium-induced contraction (10 nM: 11.1 +/- 8.51%, 100 nM: 33.4 +/- 12.7%, 100% contraction: 1.8 +/- 0.22 g), and TTX inhibits the response elicited by AM (90%). We conclude that AM modulates water and ion transport as well as bowel movement, mainly through the colonic nervous system. (C) 1998 Elsevier Science Inc.

引用

页码：1043 / 1047

页数：5

共 19 条

[1] SYNERGISTIC ACTION OF CYCLIC ADENOSINE MONOPHOSPHATE-MEDIATED AND CALCIUM-MEDIATED CHLORIDE SECRETION IN A COLONIC EPITHELIAL-CELL LINE [J].

CARTWRIGHT, CA ;

MCROBERTS, JA ;

MANDEL, KG ;

DHARMSATHAPHORN, K .

JOURNAL OF CLINICAL INVESTIGATION, 1985, 76 (05) :1837-1842

[2] EFFECTS OF ALPHA-CALCITONIN AND BETA-CALCITONIN GENE-RELATED PEPTIDES UPON ION-TRANSPORT IN RAT DESCENDING COLON [J].

COX, HM ;

FERRAR, JA ;

CUTHBERT, AW .

BRITISH JOURNAL OF PHARMACOLOGY, 1989, 97 (04) :996-998

[3] RECEPTORS FOR CALCITONIN-GENE-RELATED PEPTIDE (CGRP) IN GASTROINTESTINAL EPITHELIA [J].

COX, HM .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1995, 73 (07) :974-980

[4] SPECIFIC RECEPTORS FOR ADRENOMEDULLIN IN CULTURED RAT VASCULAR SMOOTH-MUSCLE CELLS [J].

EGUCHI, S ;

HIRATA, Y ;

KANO, H ;

SATO, K ;

WATANABE, Y ;

WATANABE, TX ;

NAKAJIMA, K ;

SAKAKIBARA, S ;

MARUMO, F .

FEBS LETTERS, 1994, 340 (03) :226-230

[5] HCO3- SECRETION BY RAT DISTAL COLON - EFFECTS OF INHIBITORS AND EXTRACELLULAR NA+ [J].

FELDMAN, GM .

GASTROENTEROLOGY, 1994, 107 (02) :329-338

[6] PROSTAGLANDIN-INDUCED AND THEOPHYLLINE-INDUCED C1 SECRETION IN RAT DISTAL COLON IS INHIBITED BY MICROTUBULE INHIBITORS [J].

GROTMOL, T ;

VANDYKE, RW .

DIGESTIVE DISEASES AND SCIENCES, 1992, 37 (11) :1709-1717

[7]

HOSOKAWA M, 1995, J PHARMACOL EXP THER, V273, P1313

[8] DISTRIBUTION AND CHARACTERIZATION OF IMMUNOREACTIVE ADRENOMEDULLIN IN HUMAN TISSUE AND PLASMA [J].

ICHIKI, Y ;

KITAMURA, K ;

KANGAWA, K ;

KAWAMOTO, M ;

MATSUO, H ;

ETO, T .

FEBS LETTERS, 1994, 338 (01) :6-10

[9] PLASMA-LEVELS OF ADRENOMEDULLIN, A NEWLY IDENTIFIED HYPOTENSIVE PEPTIDE, IN PATIENTS WITH HYPERTENSION AND RENAL-FAILURE [J].

ISHIMITSU, T ;

NISHIKIMI, T ;

SAITO, Y ;

KITAMURA, K ;

ETO, T ;

KANGAWA, K ;

MATSUO, H ;

OMAE, T ;

MATSUOKA, H .

JOURNAL OF CLINICAL INVESTIGATION, 1994, 94 (05) :2158-2161

[10]

JETT MF, 1991, J PHARMACOL EXP THER, V257, P698

← 1 2 →