Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1

被引：177

作者：

Perez, OD

Mitchell, D

Jager, GC

South, S

Murriel, C

McBride, J

Herzenberg, LA

Kinoshita, S

Nolan, GP ^{[1
]}

机构：

[1] Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Baxter Lab Genet Pharmacol, Sch Med, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Mol Pharmacol, Sch Med, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 USA

[5] Stanford Univ, Dept Immunol & Rheumatol, Sch Med, Stanford, CA 94305 USA

[6] Univ Texas, SW Med Ctr, Dept Dermatol, Ctr Immunol, Dallas, TX 75390 USA

来源：

NATURE IMMUNOLOGY | 2003年 / 4卷 / 11期

关键词：

D O I：

10.1038/ni984

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Leukocyte functional antigen 1 (LFA-1), with intercellular adhesion molecule ligands, mediates T cell adhesion, but the signaling pathways and functional effects imparted by LFA-1 are unclear. Here, intracellular phosphoprotein staining with 13-dimensional flow cytometry showed that LFA-1 activation induced phosphorylation of the 2 integrin chain and release of Jun-activating binding protein 1 (JAB-1), and mediated signaling of kinase Erk1/2 through cytohesin-1. Dominant negatives of both JAB-1 and cytohesin-1 inhibited interleukin 2 production and impaired T helper type 1 differentiation. LFA-1 stimulation lowered the threshold of T cell activation. Thus, LFA-1 signaling contributes to T cell activation and effects T cell differentiation.

引用

页码：1083 / 1092

页数：10

共 50 条

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