Fabrication and photoelectric response of poly(allylamine hydrochloride)/PM thin films by layer-by-layer deposition technique

被引：12

作者：

Chu, JF ^{[1
]}

Li, XC ^{[1
]}

Zhang, JP ^{[1
]}

Tang, J ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 305卷 / 01期

关键词：

bacteriorhodopsin; purple membrane; layer-by-layer; photocurrent; films;

D O I：

10.1016/S0006-291X(03)00710-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thin films of poly(allylamine hydrochloride) (PAH) and bacteriorbodopsin (bR) embedded in purple membrane (PM) have been prepared by layer-by-layer (LBL) self-assembly technique. The results obtained by UV-Vis spectroscopy and atomic force microscopy (AFM) analysis showed that the biological activity of bR was preserved and PM fragments could be well oriented onto the ITO substrate. A photo-electrochemical cell with the structure of ITO/(PAH/PM)(n)/electrolyte (0.5 M KCl)/Pt was fabricated and studied. The photocurrent peaks of (PAH/PM)(6) corresponding to light-on and light-off were about 200 and 100nA/cm(2), respectively, with the former enhanced 30% higher than that of the reference films made of (PDAC/PM)(6). (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：116 / 121

页数：6

共 26 条

[1] PHOTOPHYSICS AND MOLECULAR ELECTRONIC APPLICATIONS OF THE RHODOPSINS [J].

BIRGE, RR .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1990, 41 :683-733

[2] Photoelectric conversion of bacteriorhodopsin films fabricated by self-assembly technique [J].

Choi, HG ;

Min, JH ;

Han, KK ;

Choi, JW ;

Lee, WH .

SYNTHETIC METALS, 2001, 117 (1-3) :141-143

[3] Fuzzy nanoassemblies: Toward layered polymeric multicomposites [J].

Decher, G .

SCIENCE, 1997, 277 (5330) :1232-1237

[4] THE COLLOID-CHEMICAL APPROACH TO NANOSTRUCTURED MATERIALS [J].

FENDLER, JH ;

MELDRUM, FC .

ADVANCED MATERIALS, 1995, 7 (07) :607-632

[5] Photoelectric properties of oriented bacteriorhodopsin/polycation multilayers by electrostatic layer-by-layer assembly [J].

He, JA ;

Samuelson, L ;

Li, L ;

Kumar, J ;

Tripathy, SK .

JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (36) :7067-7072

[6] Oriented bacteriorhodopsin/polycation multilayers by electrostatic layer-by-layer assembly [J].

He, JA ;

Samuelson, L ;

Li, L ;

Kumar, J ;

Tripathy, SK .

LANGMUIR, 1998, 14 (07) :1674-1679

[7] ROLE OF WATER IN BACTERIORHODOPSINS CHROMOPHORE - RESONANCE RAMAN-STUDY [J].

HILDEBRANDT, P ;

STOCKBURGER, M .

BIOCHEMISTRY, 1984, 23 (23) :5539-5548

[8] STRUCTURAL AND SPECTROSCOPIC CHARACTERISTICS OF BACTERIORHODOPSIN IN AIR-WATER-INTERFACE FILMS [J].

HWANG, SB ;

KORENBROT, JI ;

STOECKENIUS, W .

JOURNAL OF MEMBRANE BIOLOGY, 1977, 36 (2-3) :115-135

[9] STUDY OF PHOTOCYCLE AND SPECTRAL PROPERTIES OF BACTERIORHODOPSIN IN LANGMUIR-BLODGETT-FILMS [J].

IKONEN, M ;

PELTONEN, J ;

VUORIMAA, E ;

LEMMETYINEN, H .

THIN SOLID FILMS, 1992, 213 (02) :277-284

[10] ORIENTED PURPLE-MEMBRANE FILMS AS A PROBE FOR STUDIES OF THE MECHANISM OF BACTERIORHODOPSIN FUNCTIONING .1. THE VECTORIAL CHARACTER OF THE EXTERNAL ELECTRIC-FIELD EFFECT ON THE DARK STATE AND THE PHOTOCYCLE OF BACTERIORHODOPSIN [J].

KONONENKO, AA ;

LUKASHEV, EP ;

MAXIMYCHEV, AV ;

CHAMOROVSKY, SK ;

RUBIN, AB ;

TIMASHEV, SF ;

CHEKULAEVA, LN .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 850 (01) :162-169

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