Lipid rafts associate with intracellular B cell receptors and exhibit a B cell stage-specific protein composition

被引:66
作者
Mielenz, D
Vettermann, C
Hampel, M
Lang, C
Avramidou, A
Karas, M
Jäck, HM
机构
[1] Univ Erlangen Nurnberg, Div Mol Immunol, Dept Internal Med 3, Nikolaus Fiebiger Ctr Mol Med, D-91054 Erlangen, Germany
[2] Univ Frankfurt, Inst Pharmaceut Chem, D-6000 Frankfurt, Germany
关键词
D O I
10.4049/jimmunol.174.6.3508
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Lipid rafts serve as platforms for BCR signal transduction. To better define the molecular basis of these membrane microdomains, we used two-dimensional gel electrophoresis and mass spectrometry to characterize lipid raft proteins from mature as well as immature B cell lines. Of 51 specific raft proteins, we identified a total of 18 proteins by peptide mass fingerprinting. Among them, we found vacuolar ATPase subunits alpha-1 and beta-2, vimentin, gamma-actin, mitofilin, and prohibitin. None of these has previously been reported in lipid rafts of B cells. The differential raft association of three proteins, including a novel potential signaling molecule designated swiprosin-1, correlated with the stage-specific sensitivity of B cells to BCR-induced apoptosis. In addition, MHC class II molecules were detected in lipid rafts of mature, but not immature B cells. This intriguing finding points to a role for lipid rafts in regulating Ag presentation during B cell maturation. Finally, a fraction of the BCR in the B cell line CH27 was constitutively present in lipid rafts. Surprisingly, this fraction was neither expressed at the cell surface nor fully O-glycosylated. Thus, we conclude that partitioning the BCR into lipid rafts occurs in the endoplasmic reticulum/cis-Golgi compartment and may represent a control mechanism for surface transport.
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收藏
页码:3508 / 3517
页数:10
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