Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium

被引：192

作者：

Pang, Xiubing ^{[1
]}

Yao, Wenbing ^{[1
]}

Yang, Xiaobing ^{[1
]}

Xie, Chen ^{[1
]}

Liu, Dong ^{[1
]}

Zhang, Jian ^{[1
]}

Gao, Xiangdong ^{[1
]}

机构：

[1] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2007年 / 70卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Flammulina velutipes; polysaccharide; mycelium; structure; D-glucan; hepatocyte;

D O I：

10.1016/j.carbpol.2007.04.010

中图分类号：

O69 [应用化学];

学科分类号：

081704 [应用化学];

摘要：

A water-soluble polysaccharide named as FVP2 has been isolated from Flammulina velutipes mycelium by hot water extraction, anion-exchange and gel-permeation chromatography. Structure study shows FVP2 is an alpha-(1 -> 4)-D-glucan, with a single alpha-D-glucan at the C-6 position approximately every seven residues, along the main chain. The weight-average molecular weight is 1.89 x 104 Da. Its biological activity was tested on hepatocytes. In vitro study indicates FVP2 can enhance the livability of primary culture of mouse hepatocytes and decrease the release of ALT as well as apoptosis of hepatocytes after CCl4 intoxication. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：291 / 297

页数：7

共 31 条

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