Quality control of PCR primers used in multiplex STR amplification reactions

被引：18

作者：

Butler, JM

Devaney, JM

Marino, MA

Vallone, PM

机构：

[1] Natl Inst Stand & Technol, Div Biotechnol, Gaithersburg, MD 20899 USA

[2] Transgenom Inc, Gaithersburg, MD 20878 USA

来源：

FORENSIC SCIENCE INTERNATIONAL | 2001年 / 119卷 / 01期

关键词：

short tandem repent DNA typing; oligonucleotide quality control; HPLC; mass spectrometry; multiplex PCR amplification;

D O I：

10.1016/S0379-0738(00)00412-6

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification reactions where multiple STR loci are simultaneously copied. Commercially available kits are now widely used for STR amplification and subsequent DNA typing. We present here the use of high performance liquid chromatography (HPLC) and time-of-flight mass spectrometry (TOF-MS) methods for characterization of commercially available STR kits. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：87 / 96

页数：10

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