Mapping quantitative trait loci in European sea bass (Dicentrarchus labrax):: The BASSMAP pilot study

被引：43

作者：

Chatziplis, Dimitrios ^{[1
]}

Batargias, Costas ^{[2
]}

Tsigenopoulos, Constantinos S. ^{[3
]}

Magoulas, Antonios ^{[3
]}

Kollias, Spyros ^{[3
]}

Kotoulas, Georgios ^{[3
]}

Volckaert, Filip A. M. ^{[4
]}

Haley, Chris S. ^{[5
]}

机构：

[1] Aristotle Univ Thessaloniki, Fac Agr, Dept Anim Prod, GR-54006 Thessaloniki, Greece

[2] NIREUS CHIOS AQUACULTURE SA, GR-19400 Chios, Greece

[3] Hellen Ctr Marine Res, Dept Genet & Mol Biotechnol, Inst Marine Biol & Genet, GR-71500 Iraklion, Greece

[4] Katholieke Univ Leuven, Lab Anim Divers & Systemat, B-3000 Louvain, Belgium

[5] Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland

来源：

AQUACULTURE | 2007年 / 272卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

QTL; genetic parameters; microsatellite; heritability; European sea bass; Dicentrarchus labrax;

D O I：

10.1016/j.aquaculture.2007.08.022

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

There are great opportunities for genetic improvement in recently domesticated aquaculture species. However, the lack of appropriate tools limits the application of advanced techniques including the mapping of quantitative trait loci (QTL) and marker-assisted selection (MAS). The recent development of a genetic linkage map for the European sea bass allows the application of such methods for the first time in this species. We report a pilot trial of QTL mapping in a commercial sea bass population as a precursor to the application of MAS. Fertilized eggs collected on a single spawning day produced a population composed of a few large families. Fish were grown under commercial conditions, slaughtered at approximately 470 g and measurements were taken for several traits. Parentage analysis showed that the population consisted of 26 full sib families, with a common dam and several sires. Seven microsatellite markers spanning the largest linkage group (LG1) were then genotyped in 27 parents and their 422 progeny, in order to perform a QTL scan using half-sib interval mapping approaches. Significant QTL affecting six morphometric traits including length and depth, are reported. The success of the procedure demonstrates that QTL analysis can be applied in this species and will contribute to the study and future improvement of traits associated with production, profitability and sustainability. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：S172 / S182

页数：11

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