Early detection of cacao swollen shoot virus using the polymerase chain reaction

被引：23

作者：

Muller, E

Jacquot, E

Yot, P

机构：

[1] CIRAD, AMIS, Crop Protect Programme, Virol Unit, F-34032 Rennes, France

[2] CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France

来源：

JOURNAL OF VIROLOGICAL METHODS | 2001年 / 93卷 / 1-2期

关键词：

cacao swollen shoot virus; early diagnosis; PCR; badnavirus; PCR-inhibitory components;

D O I：

10.1016/S0166-0934(00)00241-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymerase chain reaction assay was developed which allows early detection of cacao swollen shoot virus (CSSV) in DNA extracts from cacao plantlets agroinoculated with the Togolese isolate Agou 1. The primers used were derived from badnavirus conserved sequences and nucleic acid was extracted with the Plant DNeasy(R); extraction kit (Qiagen). CSSV genome was detectable between 6 and 20 days after inoculation. The first leaf symptoms appeared after 4 weeks and the first shoot swelling symptoms after 8 weeks. (C) 2001 Elsevier Science B.V. All rights reserved.

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收藏

页码：15 / 22

页数：8

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