Speciation analysis of mercury contaminants in water samples by RP-HPLC after solid phase extraction on modified C18 extraction disks with 1,3-bis(2-cyanobenzene)triazene

A highly sensitive and accurate method for preconcentration and determination of ultra trace amounts of inorganic mercury and organomercuty compounds in different water samples is proposed. The preconcentration is achieved using octadecyl silica (C-18) extraction disks modified with 1,3-bis(2-cyanobenzene)triazene (CBT). The retained analytes as their triazenide complexes on the solid phase was eluted with 10 ml acetonitrile and measured by reversed-phase high-performance liquid chromatography (RP-HPLC). Type and amount of eluent, pH, amount of CBT, flow rates of sample solution and eluent have been optimized in order to obtain quantitative recovery of the anallytes. The effect of interfering ions, such as Cu2+, Mn2+, Fe2+, Al3+, Zn2+, Cd2+, Ca2+, Mg2+, Ba2+, Ph2+, K+ and Na+ usually present in water samples on the recovery of the analytes has also been investigated. The enrichment factor of 100 was obtained for all mercury species and the analytical detection limits of phenylmercury, methylmercury and Hg2+ were found as 0.8, 1.0 and 1.3 ng l(-1), respectively. Stability of mercury species after extraction on the modified disks was studied and the results showed that complexes collected on the disks were stable for at least 5 days. The proposed method has been applied to the quantitative determination of mercury species in natural and synthetic water samples with recoveries more than 90%. (C) 2008 Elsevier B.V. All rights reserved.