Biodistribution of phosphodiester and phosphorothioate siRNA

被引：198

作者：

Braasch, DA

Paroo, Z

Constantinescu, A

Ren, G

Öz, OK

Mason, RP

Corey, DR

机构：

[1] Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA

[2] Univ Texas, SW Med Ctr, Dept Biochem, Dallas, TX 75390 USA

[3] Univ Texas, SW Med Ctr, Dept Radiol, Dallas, TX 75390 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 05期

关键词：

siRNA; phosphorothioate; biodistribution; planar imaging;

D O I：

10.1016/j.bmcl.2003.12.074

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Short interfering RNAs (siRNAs) are valuable tools for analyzing protein function in mammalian cell culture. This success has led to high expectations for in vivo and therapeutic applications. However, the pharmacokinetic properties of siRNA are not known. Here we report the biodistribution of a phosphodiester (PO) siRNA duplex and examine the effect of phosphorothioate (PS) linkages. Our findings indicate that biodistribution of siRNA is similar to that for single-stranded antisense oligonucleotides and offer insights for use of siRNA in vivo. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1139 / 1143

页数：5

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