Detection of nucleotide variability in rpoB in both rifampin-sensitive and rifampin-resistant strains of Chlamydia trachomatis

被引：26

作者：

Dreses-Werringloer, U

Padubrin, I

Köhler, L

Hudson, AP

机构：

[1] Wayne State Univ, Sch Med, Dept Immunol & Microbiol, Detroit, MI 48201 USA

[2] Hannover Med Sch, Div Rheumatol, Dept Internal Med, D-3000 Hannover, Germany

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 07期

关键词：

D O I：

10.1128/AAC.47.7.2316-2318.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 656-bp PCR fragment from rpoB was sequenced from five rifampin-resistant Chlamydia trachomatis variants selected in vitro from a wild-type parent with a surprising level of genetic variability in this region. Three variants (MIC, 4 mug/ml) showed Ala522-->Val in cluster I (codons 507 to 533), which harbors mutations in most rifampin-resistant bacteria. Two high-level resistance variants (MICs, 64 and 256 mug/ml) showed His526-->Tyr in cluster I with additional genetic variation, some of which resulted in amino acid substitutions. None of the latter was situated in clusters related to rifampin resistance in other bacteria.

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页码：2316 / 2318

页数：3

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