Genotyping of verocytotoxin-producing Escherichia coli O157:: Comparison of isolates of a prevalent phage type by fluorescent amplified-fragment length polymorphism and pulsed-field gel electrophoresis analyses

被引：15

作者：

Smith, D

Willshaw, G

Stanley, J

Arnold, C

机构：

[1] Cent Publ Hlth Lab, Mol Biol Unit, Div Virus Reference, London NW9 5HT, England

[2] Cent Publ Hlth Lab, Lab Enteric Pathogens, London NW9 5HT, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 12期

关键词：

D O I：

10.1128/JCM.38.12.4616-4620.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We applied the high-resolution genotyping technique fluorescent amplified-fragment length polymorphism (FAFLP) analysis to 71 isolates of a single phage type (PT8) of pulsed-field gel electrophoresis (PFGE)-characterized verocytotoxin-producing Escherichia coli O157. Twenty-seven similar, but not identical, groupings were defined by both FAFLP analysis and the PFGE profiles. Given the FAFLP analysis conditions described here, these two methods exhibited equivalent discriminatory powers.

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页码：4616 / 4620

页数：5

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