Ultra-deep two-photon fluorescence excitation in turbid media

被引：111

作者：

Beaurepaire, E ^{[1
]}

Oheim, M ^{[1
]}

Mertz, J ^{[1
]}

机构：

[1] Ecole Super Phys & Chim Ind, INSERM, Lab Neurophysiol & Nouvelles Microscopies, EPI 0002, F-75005 Paris, France

来源：

OPTICS COMMUNICATIONS | 2001年 / 188卷 / 1-4期

关键词：

Fluorescence - Laser pulses - Optical resolving power - Photons;

D O I：

10.1016/S0030-4018(00)01156-1

中图分类号：

O43 [光学];

学科分类号：

070207 [光学]; 0803 [光学工程];

摘要：

An important application of two-photon excited fluorescence (TPEF) microscopy is to provide high-resolution images from deep within scattering media. We investigate strategies to further improve TPEF penetration depth by considering the effects of scattering on fluorescence generation and collection separately. In particular, we demonstrate that the redistribution of laser power into higher energy pulses by means of a regenerative amplifier improves the TPEF depth penetration by two to three excitation scattering mean free paths. (C) 2001 Published by Elsevier Science B.V.

引用

页码：25 / 29

页数：5

共 8 条

[1]

Beaurepaire Emmanuel, 2000, European Biophysics Journal, V29, P388

[2]

Monte Carlo analysis of two-photon fluorescence imaging through a scattering medium [J].

Blanca, CM ;

Saloma, C .

APPLIED OPTICS, 1998, 37 (34) :8092-8102

[3]

Denk Winfried, 1995, P445

[4]

Influence of optical properties on two-photon fluorescence imaging in turbid samples [J].

Dunn, AK ;

Wallace, VP ;

Coleno, M ;

Berns, MW ;

Tromberg, BJ .

APPLIED OPTICS, 2000, 39 (07) :1194-1201

[5]

Molecular photodynamics involved in multi-photon excitation fluorescence microscopy [J].

Mertz, J .

EUROPEAN PHYSICAL JOURNAL D, 1998, 3 (01) :53-66

[6]

Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability [J].

Squirrell, JM ;

Wokosin, DL ;

White, JG ;

Bavister, BD .

NATURE BIOTECHNOLOGY, 1999, 17 (08) :763-767

[7]

In vivo dendritic calcium dynamics in neocortical pyramidal neurons [J].

Svoboda, K ;

Denk, W ;

Kleinfeld, D ;

Tank, DW .

NATURE, 1997, 385 (6612) :161-165

[8]

Effect of scattering on nonlinear optical scanning microscopy imaging of highly scattering media [J].

Ying, JP ;

Liu, F ;

Alfano, RR .

APPLIED OPTICS, 2000, 39 (04) :509-514

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